Research Progress on Interference of Endogenous Factors in Detection of Serum Cardiac Troponin I by Sandwich Antibody Immunoassay and Its Solutions
10.3969/j.issn.1671-7414.2024.01.035
- VernacularTitle:内源性因素对抗体夹心免疫法检测血清心肌肌钙蛋白I的干扰及解决方案研究进展
- Author:
Chengshan HE
1
;
Yang LIU
;
Zheng XU
;
Xiudi JIANG
;
Zhicheng LU
Author Information
1. 上海中医药大学附属第七人民医院医学检验科,上海 200137
- Keywords:
cardiac troponin I;
antibodies;
sandwich immunoassay;
interference;
endogenous factor
- From:
Journal of Modern Laboratory Medicine
2024;39(1):186-191
- CountryChina
- Language:Chinese
-
Abstract:
Cardial troponin I(cTnI)is the preferred serological marker for the diagnosis of myocardial injury.cTnI detection is based on antibody sandwich immunoassay.The epitopes of cTnI antigen targeted by detecting and capturing antibodies in different detection reagents are inconsistent,which easily leads to the heterogeneity of cTnI detection results.Endogenous interfering factors such as cTnI autoantibody,heterophile antibody,rheumatoid factor,ect,which can seriously interfere with the results of cTnI detection,and affecting the clinical diagnosis,treatment and prognosis of myocardial injury diseases.In this paper,the research progress of antibody sandwich immunoassay for cTnI and interference of endogenous factors on cTnI detection and solutions are reviewed to provide theoretical basis for differential diagnosis of abnormal cTnI detection results in clinical practice.
