Establish an Anti-biotin Interference Method to Detect Serum β-hCG and Prog Levels Based on BAS Immunoassays
10.3969/j.issn.1671-7414.2024.01.026
- VernacularTitle:建立一种基于BAS化学发光技术检测血清β-hCG,Prog水平的抗生物素干扰方法
- Author:
Long CHEN
1
;
Xiangwu ZHENG
Author Information
1. 通用环球西安北环医院检验科,西安 710032
- Keywords:
biotin;
streptavidin coated magnetic micro particles;
human chorionic gonadotropin β subunit;
progesterone
- From:
Journal of Modern Laboratory Medicine
2024;39(1):141-145
- CountryChina
- Language:Chinese
-
Abstract:
Objective A simple and effective anti-biotin interference method was established to detect human chorionic gonadotropin β subunit(β-hCG)and progesterone(Prog)in BAS immunoassays.Methods Using two different concentrations of streptavidin magnetic particles(M)to detect high,medium and low levels of β-hCG and Prog serum with different biotin concentrations,the anti-biotin interference ability of two kinds of M and the accuracy of high concentration M to detect β-hCG and Prog were evaluated through recovery test when the calibration curve of low concentration M is adopted.Results ①The anti-biotin interference ability of β-hCG and Prog were 100 and 25 ng/ml respectively at low concentration M(0.72 mg/ml),and were 500 and 50 ng/ml respectively at high concentration M(1.44 mg/ml).②When using the same calibration curve as low concentration M,the recovery rate of high concentration M for β-hCG at three levels with biotin below 500 ng/ml were between 90%and 110%,for Prog with high and medium levels of biotin below 50 ng/ml,the recovery rate were between 90%～110%.Conclusion When detecting serum nterference ability of β-hCG mmunoassays,the method of high concentration M(1.44 mg/mL)is a simple,effective and reliable anti-biotin interference program.
