Rapid Analysis of Chemical Constituents in Shenxianshengmai Oral Liquid by UHPLC-Q Exactive Focus MS/MS
- VernacularTitle:参仙升脉口服液化学成分的UHPLC-Q Exactive Focus MS/MS快速分析
- Author:
Shiyu ZONG
1
;
Zhibiao DI
;
Qiqi LIU
;
Zhuangzhuang HUANG
;
Hong ZHANG
;
Ye LI
;
Feng LIU
;
Yang LIU
Author Information
- Keywords: Shenxianshengmai oral liqid; UHPLC-Q Exactive Focus MS/MS; Chemical constituents; Flavonoids; Organic acids
- From: World Science and Technology-Modernization of Traditional Chinese Medicine 2024;26(1):218-228
- CountryChina
- Language:Chinese
- Abstract: Objective To explore the chemical basis of Shenxianshengmai oral liquid.Method UHPLC-Q Exactive Focus MS/MS technology was used to identify the chemical components of Shenxianshengmai oral liquid.The chromatographic separation was performed on a Thermo Accucore aQ C18 column(150 mm×2.1 mm,2.6 μm)with mobile phase gradient elution of 0.1%formic acid aqueous solution(A)and methanol(B)for 0-13 min,5%-60%B;13-27 min,60%-95%B;27-30 min,95%B,the flow rate was 0.3 mL·min-1,and the column temperature was at 30℃.The mass spectrometry was performed by heating electrospray ionization(H-ESI)with positive and negative ion scanning modes.The scanning range was m/z 120-1800,and the collision energies were 30 eV,50 eV and 70 eV.Result A total of 160 components were identified,including 29 flavonoids,24 organic acids,21 alkaloids,19 terpenoids,15 phenylpropanoids,12 saponins and 40 other components.Six chemical constituents(rutin,psoralenoside,isopsoralenoside,psoralen,isopsoralen and bakuchiol)were confirmed by comparison with reference substances.Conclusion In this study,an UHPLC-Q Exactive Focus MS/MS method has been established for accurate,rapid and systematic identification of the constituents in Shenxian Shengmai oral liquid,which provides an important basis for clarifying the chemical basis and quality control.
