To Explore the Effect and Mechanism of Shenghui Granule on EC and CA1 Regions of AD Model Rats Based on p38 MAPK Signal Pathway
- VernacularTitle:基于p38 MAPK信号通路探讨生慧颗粒对AD模型大鼠EC与CA1区影响及作用机制
- Author:
Zefei LI
1
,
2
;
Binbin ZHAO
;
Heyuan SHI
;
Ping WANG
Author Information
- Keywords: Shenghui granules; Alzheimer's disease; Entorhinal cortex; Hippocampus CA1; c-Fos; p38 MAPK
- From: World Science and Technology-Modernization of Traditional Chinese Medicine 2024;26(1):157-166
- CountryChina
- Language:Chinese
- Abstract: Objective To observe the effect of Shenghui Granule on EC and CA1 regions of scopolamine-induced dementia rats and explore its mechanism based on p38 MAPK signal pathway.Methods 40 SD rats were randomly divided into four groups(blank group,model group,Shenghui granule group,donepezil group),and were treated with scopolamine.Morris water maze and open field test were used to evaluate the cognition and anxiety behavior of rats.The nerve injury of EC and CA1 was observed by HE staining.The activity of neurons in EC and CA1 regions was observed by c-Fos immunofluorescence staining.Western blot was used to detect p38 MAPK pathway related proteins.Results The behavioral experiment found that Shenghui Granule could improve the cognitive impairment and anxiety-like behavior of AD model rats.The results of HE staining showed that Shenghui granules had protective effects on EC and CA1 regions.The results of c-Fos immunofluorescence staining showed that Shenghui granules could increase the activity of neurons in EC and CA1 regions.Western blot results showed that Shenghui Granule could down-regulate the expression of Bax,reduce the levels of phosphorylated p38 and Tau,and increase the expression of Bcl-2.Conclusion Shenghui granule has protective effect on EC and CA1 regions of AD model rats,and may play a therapeutic role through p38 MAPK signal pathway.
