Efficacy Evaluation and Mechanism Research of Qi-Shen-Yi-Zhi Formula in Improving the Learning and Memory Ability of Aβ1-42 Hippocampus Injection Mice
- VernacularTitle:杞参益智方改善Aβ1-42海马区注射小鼠学习与记忆能力的效用评价与作用机制研究
- Author:
Ziqiang ZHU
1
;
Yunqing LU
;
Jiani ZHENG
;
Cheng CAO
;
Yang CHEN
;
Jiaxiang TONG
;
Xuan LI
;
Sheng GUO
;
Jin'ao DUAN
;
Yue ZHU
Author Information
- Keywords: Qi-Shen-Yi-Zhi formula; Alzheimer's disease; Oxidative stress; Neuronal inflammation
- From: World Science and Technology-Modernization of Traditional Chinese Medicine 2024;26(1):40-47
- CountryChina
- Language:Chinese
- Abstract: Objective Evaluation of the effect and mechanism research of Qi-Shen-Yi-Zhi formula on improving learning and memory ability in mice injected with Aβ1-42 in hippocampus.Methods Alzheimer's disease model mice were constructed by injecting β amyloid peptide 1-42 into hippocampus and treated with water extracts of Qi-Shen-Yi-Zhi formula.The cognitive abilities of mice were assessed using Morris water maze and Y maze tests,which measure learning and memory capabilities.HE staining was used to observe the damage and TUNEL method was used to determine apoptosis of hippocampus.Detection of the expression of oxidative factors,inflammatory factors,and related antioxidant proteins and apoptotic proteins in the hippocampal tissue of a mouse model of dementia.Results Both high-dose and low-dose groups of Qi-Shen-Yi-Zhi formula significantly improved cognitive dysfunction in mice injected with Aβ1-42 in hippocampus,and attenuated the damage and apoptosis of the hippocampus.It also inhibited oxidative stress and downregulated the expressions of inflammatory factors IL-6,IL-1β and TNF-a,increased the expression of antioxidant proteins Nrf2 and HO-1,and regulated the expressions of apoptotic proteins Caspase-9,Caspase-3,Bax and Bcl-2.Conclusion Qi-Shen-Yi-Zhi formula improves the learning and memory abilities of mice injected with Aβ1-42 in hippocampus,which might be related to the attenuation of oxidative stress and neuronal inflammation of hippocampus.
