A Study of Plasma Exosome Protein Spectrum in Coronary Heart Disease Patients with Phlegm Turbidity Syndrome and Healthy People
- VernacularTitle:冠心病痰浊证与健康人血浆外泌体蛋白质谱研究
- Author:
Xueping ZHU
1
;
Yan ZHOU
;
Guozhen YUAN
;
Qiulei JIA
;
Shuqing SHI
;
Bai DU
;
Yuanhui HU
Author Information
- Keywords: Coronary heart disease; Phlegm-turbidity syndrome; Exosome; Protein spectrum
- From: World Science and Technology-Modernization of Traditional Chinese Medicine 2023;25(12):3928-3943
- CountryChina
- Language:Chinese
- Abstract: Objective To explore the specific plasma exosomal protein expression in Coronary Heart Disease(CHD)with phlegm-turbidity syndrome(PTS),so as to provide biological reference for the diagnosis of CHD with PTS.Methods A total of 20 CHD patients with PTS and 20 healthy subjects at the same period were included.Plasma exosomes were extracted from all subjects using the qEV size exclusion method.First,10 CHD cases with PTS and 10 healthy subjects were randomly selected,and differentially expressed proteins between the two groups were screened using label free non-targeted protein quantification analysis.Then,MRM targeting protein labeling technique was applied to verify the differentially expressed proteins in the remaining subjects.Results Compared with healthy controls,214 differentially expressed plasma exosomal proteins(61 up-regulated and 153 down-regulated)were found in CHD patients with PTS,mainly related to cholesterol metabolism,complement and coagulation cascade,and immune effects.ANXA6,C4BPB,F8,CFB,APOE,C9,and CLU proteins were further validated by MRM targeting protein.Conclusion CHD patients with PTS had differences in plasma exosomal protein expression from healthy controls,and the differential proteins are mainly related to cholesterol metabolism,complement,and the coagulation cascade.
