Molecular Mechanism of Prepared Radix Polygoni Multiflori in Treatment of Non-alcoholic Fatty Liver Disease Based on Network Pharmacology and Animal Experiments
10.11842/wst.20221130003
- VernacularTitle:基于网络药理学方法和动物实验探讨制何首乌治疗非酒精性脂肪肝病的作用机制
- Author:
Huiru YANG
1
;
Changyudong HUANG
;
Liying ZHU
;
Chengcheng LI
;
Yongjie XU
;
Xing LI
;
Wei PAN
;
Zulong ZHANG
Author Information
1. 贵州医科大学附属医院 贵阳 550004
- Keywords:
Radix Polygoni Multiflori;
Nonalcoholic fatty liver disease;
Network pharmacology;
Fatty acid synthase
- From:
World Science and Technology-Modernization of Traditional Chinese Medicine
2023;25(11):3678-3691
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore and validate the mechanism of Prepared Radix Polygoni Multiflori in the treatment of NAFLD based on network pharmacology and animal non-alcoholic fatty liver disease(NAFLD)model experiments.Methods Consult the literature to compare the differences between Radix Polygoni Multiflori and Prepared Radix Polygoni Multiflori(PRPM).Herb database and SwissADME database were used to screen the active ingredients of Prepared Radix Polygoni Multiflori,SwissTargetPrediction database was used to predict its targets,OMIM,DISGENET and GEENCARDS databases were used to screen the NAFLD-related targets,conduct GO functional enrichment analysis and KEGG pathway enrichment analysis.The active ingredient-target-KEGG signaling pathway-NAFLD network was mapped later.The mice with NAFLD were treated with Prepared Radix Polygoni Multiflori by gavage for 8 weeks;serum triglyceride level and alanine aminotransferase(ALT)activity were measured;the liver lesions were observed by HE staining;the potential mechanism of action of Radix Polygoni Multiflori in the treatment of NAFLD was verified by Western blot.Results The differences between Radix Polygoni Multiflori and PRPM were consulted.Six pharmacological components and 32 potential action targets of Radix Polygoni Multiflori for the treatment of NAFLD were screened by network pharmacology,GO and KEGG pathways were enriched to lipid and atherosclerosis-related pathways,AMPK signaling pathway,etc.;HE staining verified that Prepared Radix Polygoni Multiflori has the function of improving NAFLD and is associated with the alteration of FASN,ACC,SCD protein of AMPK signaling pathway.Conclusion Radix Polygoni Multiflori has the potential to improve NAFLD by regulating FASN,ACC and SCD.
