Study on the Jianpi Huatan Formula Regulating VSMC Proliferation-related miRNA in ApoE-/-AS mice Based on High-throughput Sequencing Technology
- VernacularTitle:基于高通量测序技术探讨健脾化痰方对ApoE-/-AS小鼠VSMC增殖相关miRNA的调控作用
- Author:
Miao TIAN
1
,
2
;
Lianqun JIA
;
Xing JU
;
Le GUAN
;
Yang WANG
;
Dongyu MIN
;
Gaole DU
;
Jingyu TONG
;
Guanlin YANG
Author Information
- Keywords: miRNA sequencing; Invigorating the spleen and reducing phlegm; Vascular smooth muscle cells; Atherosclerosis; Spleen dominating muscle
- From: World Science and Technology-Modernization of Traditional Chinese Medicine 2023;25(11):3646-3653
- CountryChina
- Language:Chinese
- Abstract: Objective This study used high-throughput sequencing technology to detect the regulation of JianPiHuaTan Prescription(JPHT)on the ApoE-/-mouse miRNA related to vascular smooth muscle cells proliferation and migration,verify the expression of related proteins,and explore the therapeutic effect of JPHT on atherosclerosis.Methods Ten of C57BL/6J mice were used as control group,and 30 ApoE-/-mice were randomly divided into model group,western medicine group and JPHT group.Sequencing technology was performed on aortic sample to select the differentially expressed miRNA.We screened out the tagetted miRNA related to VSMCs proliferation and migration.RT-qPCR and Western blot technology were used to test the differentially expressed miRNA and tagetted protein.Results Compared with model group,a total of 9 miRNAs were changed in JPHT group,among which 7 were up-regulated and 2 were down-regulated.The expression of miRNA-34a was up-regulated in JPHT group.The miRNA-34a and α-SMA protein expression in aorta of JPHT group were significantly different with model group by RT-qPCR and Western blot experiment(P<0.01).Conclusion Jianpi Huatan Prescription may improve ApoE-/-mice atherosclerosis through inhibitting the proliferation of vascular smooth muscle cells by regulating miRNA-34a.
