Metabolism and Excretion of Isochlorogenic Acid B in Rats by Ultra-High Performance Liquid Chromatography-Mass Spectrometry
10.11842/wst.20221005004
- VernacularTitle:基于超高效液相色谱-质谱技术的异绿原酸B大鼠体内代谢及排泄研究
- Author:
Haitao TIAN
1
;
Zhe ZHANG
;
Chunying CAI
;
Zhipeng DENG
Author Information
1. 山东中医药大学药学院 济南 250355
- Keywords:
Isochlorogenic acid B;
UHPLC-MS;
Metabolic pathways;
Excretion study
- From:
World Science and Technology-Modernization of Traditional Chinese Medicine
2023;25(11):3605-3614
- CountryChina
- Language:Chinese
-
Abstract:
Objective The metabolism and excretion of isochlorogenic acid B in rats were investigated by UHPLC-MS.Methods Feces,urine and plasma were individually collected before and at different time points after administration of 20 mg·kg-1.Post-prepared samples were analyzed by UHPLC-MS.Results According to the retention times,m/z,characteristic fragment ions and related literature,a total of 22 metabolites were detected,of which 18 metabolites were present in rat feces,3 metabolites in urine and one metabolite in plasma.The main metabolic pathways contain hydrolysis,hydrogenation,methylation,sulfation and so on.The cumulative excretion of isochlorogenic acid B and its main metabolite chlorogenic acid in feces and urine was further performed.Conclusion The metabolism and excretion of isochlorogenic acid B in rats were explored to provide experimental basis for its further research and development.
