Study on Chemotype of Tibetan Medicine"Bangjian"Multi-Origin Species Based on HPLC Fingerprint and UPLC-Q-TOF-MS
10.11842/wst.20221129015
- VernacularTitle:基于HPLC指纹图谱及UPLC-Q-TOF-MS的藏药"榜间"多基原品种化学型研究
- Author:
Jinya FAN
1
;
Cuo NAN
;
Wei DENG
;
Rui GU
;
Shihong ZHONG
;
Jinbo ZHANG
Author Information
1. 成都中医药大学药学院 成都 611137
- Keywords:
Tibetan medicine;
"Bangjian";
Chemotype;
Fingerprint;
UPLC-Q-TOF-MS;
Chemometrics
- From:
World Science and Technology-Modernization of Traditional Chinese Medicine
2023;25(11):3554-3570
- CountryChina
- Language:Chinese
-
Abstract:
Objective The HPLC fingerprints of different varieties of Tibetan medicine"Bangjian"were investigated to compare and analyse the differences in the chemical composition of different varieties and to further classify them,so as to provide reference for their quality control and safe clinical use.Methods HPLC method was used to establish the fingerprints of the different species of"Bangjian",and UPLC-Q-TOF-MS method was used to analyse the similarities and differences of the chemical components of the mainstream species and to identify the characteristic peaks.Chemometrics methods were used for the analysis including cluster analysis(HCA),principal component analysis(PCA)and orthogonal partial least squares discriminant analysis(OPLS-DA).Results A total of 11 chromatographic peaks were identified from 93 batches of"Bangjian"samples.The HCA and PCA methods were used to classify the 93 batches into 2 categories,and then OPLS-DA was used to classify them into 3 categories in more detail,while 4 main components were selected according to the principle of VIP>1.The results of UPLC-Q-TOF-MS and chemometric showed that the Tibetan medicine"Bangjian"could be divided into iridoids with benzoyl fragments represented by Gentiana szechenyii Kanitz and monocyclic iridoids represented by Gentiana veitchiorum Hemsl.according to their chemotypes.The latter could be divided into alpine gentian group subtype and multi-branch group ornate subtype according to the content of components.The results of the chemotypic classification proved that the traditional classification of the"Bangjian"has a material basis in science but were also flawed.Conclusion The present study demonstrates that the established HPLC fingerprint can be used to classify the"Bangjian"of the complex base elements effectively,which is expected to provide an effective reference for the improvement of quality standards of"Bangjian"and clinical safety medication.
