Study on the Quality Analysis Method of Schizonepeta Spike Based on Multiple Feature Components"Quality-quantity"Double Standard
10.11842/wst.20221031007
- VernacularTitle:基于多个特征成分"质-量"双标的荆芥穗质量分析方法研究
- Author:
Mengnan JIA
1
;
Tianjiao LI
;
Yongrui BAO
;
Shuai WANG
;
Xiansheng MENG
Author Information
1. 辽宁中医药大学药学院 大连 116600
- Keywords:
Schizonepeta Spike;
Reference crude drug;
Quality control;
Specific chromatogram;
Pulegone
- From:
World Science and Technology-Modernization of Traditional Chinese Medicine
2023;25(11):3506-3514
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a"quality-quantity"double standard control method of Schizonepeta Spike based on the reference material without relying on multiple reference substances.Methods HPLC technology was used to establish the characteristic atlas of Schizonepeta Spike based on the reference traditional Chinese medicinal materials.The chemical constituents of common peaks were identified by Q-TOF-MS technology,and the authenticity of Schizonepeta Spike was determined by the similarity of characteristic peaks of the reference traditional Chinese medicinal materials and the tested traditional Chinese medicinal materials.The relative content of each characteristic peak in different batches of the tested traditional Chinese medicinal materials was calculated by accurate quantition of pulegone.The"Average value-Standard deviation"was taken as the lower limit of the relative content of characteristic peak chemical components relative to pulegone,and the quality of Schizonepeta Spike was determined according to the lower limit of the relative content of characteristic peak.Results The characteristic atlas and content determination method met the requirements of methodology investigation.A total of 11 common chromatographic peaks were determined,and 6 chemical components were identified,which were luteolin,hesperidin,luteolin-7-O-glucuronide,luteolin,geranyl,pulegone.The similarity between the tested traditional Chinese medicinal materials and reference traditional Chinese medicinal materials was greater than 0.90.The lower limit of relative content of chemical components of Schizonepeta Spike characteristic peak was defined.Conclusion The method can identify Schizonepeta Spike clearly and quickly without many reference substances,and provide reference for quality control of Schizonepeta Spike.
