Proteomics Changes of Mice Lungs and Colon during Lipopolysaccharide-Induced Lung Injury and the Effect of Platycodonis Radix and Rhei Radix et Rhizoma
10.11842/wst.20221207008
- VernacularTitle:内毒素所致肺损伤小鼠肺结肠蛋白质组学变化及其桔梗大黄的作用
- Author:
Fan LEI
1
;
Hong SUN
;
Zongyuan LIU
;
Weihua WANG
;
Weidong XIE
;
Xinchun LUO
;
Rui LUO
;
Dongming XING
;
Lijun DU
Author Information
1. 清华大学生命科学学院 北京 100084
- Keywords:
Lung;
Colon;
Lipopolysaccharide;
Proteome;
Platycodonis Radix;
Rhei Radix et Rhizoma
- From:
World Science and Technology-Modernization of Traditional Chinese Medicine
2023;25(9):3132-3145
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the proteomic profiling of lung and colon during lung injury induced by lipopolysaccharide(LPS).Methods Mice were divided into four groups:the control,LPS,LPS+ Platycodonis Radix(PR)and LPS+ Rhei Radix et Rhizoma(RRR).LPS was injected into the lungs through trachea,and the drugs were given by intragastric injection.The mice were weighed,the faeces of each mouse were determined,and the lungs and colon were isolated for analysis of pathophysiological changes and proteomics.Results ①After 7 days of LPS,the weight of mice decreased,the lung showed inflammatory changes,and the faeces increased.Both PR and RRR can improve the inflammation.②There are lot of proteins was increased in lung mainly involved in gene transcription and in colon mainly involved in mitochondrial,endoplasmic reticulum and metabolism,etc.The up-regulated proteins shared by both lung and colon were involved in myoprotein contraction.PR can inhibit the up-regulated protein more than RRR in lung.③There are large number of proteins were down-regulated in lung involved in cell membrane and in colon involved in nucleic acid binding and ATP binding.The down-regulated proteins shared by both lung and colon were involved in endoplasmic reticulum,nucleic acid binding and cell membrane,etc.The down-regulated proteins in lung by PR are more than those by RRR,which is involved in endoplasmic reticulum,cell membrane,etc.Conclusion LPS-induced lung injury can cause changes in the expression of protein in lung and colon proteins,and the increase in the expression of myoprotein contraction genes may be one of the molecular mechanisms related to lung and colon.
