Effect of Jiawei Bazhen Yimu Capsule on Serum Metabolomics in Premature Ovarian Failure Rats Based on UPLC-Q-TOF-MS
10.11842/wst.20221011004
- VernacularTitle:基于UPLC-Q-TOF-MS研究加味八珍益母胶囊对卵巢早衰大鼠血清代谢组学的影响
- Author:
Liying HAN
1
;
Shuai WANG
;
Yongrui BAO
;
Tianjiao LI
;
Lingwei ZHANG
;
Xiansheng MENG
Author Information
1. 辽宁中医药大学药学院 大连 116600
- Keywords:
Jiawei Bazhen Yimu Capusule;
Premature ovarian failure;
Metabonomics;
UPLC-Q-TOF-MS
- From:
World Science and Technology-Modernization of Traditional Chinese Medicine
2023;25(9):3125-3131
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the mechanism of Jiawei Bazhen Yimu Capsule on premature ovarian failure rats from the perspective of metabolomics.Methods Female SD rats were randomly divided into sham operation group,model group and Jiawei Bazhen Yimu Capsule low,medium and high dose groups,with 10 rats in each group.The model of premature ovarian failure was replicated by removing bilateral ovaries of rats and administered intragastrically once a day for 21 days.The serum samples of rats in each group were analyzed by ultra-high performance liquid chromatography-quadrupole-time of flight tandem mass spectrometry(UPLC-Q-TOF-MS).Combined with multivariate statistical analysis,the effects of Jiawei Bazhen Yimu Capsule on differential metabolites in rats with premature ovarian failure were investigated.The differential metabolites identified by MELIN database or KEGG database were imported into Metaboanalyst 5.0 online platform for metabolic pathway analysis.Results A total of 18 potential differential metabolites were screened and identified.Most of the differential metabolites showed a good callback trend after intragastric administration of Jiawei Bazhen Yimu Capsule.These 18 differential metabolites were enriched into 2 metabolic pathways(Pathway Impact>0.1),which were glycerophospholipid metabolism and arachidonic acid metabolism pathways.Conclusion The therapeutic effect of Jiawei Bazhen Yimu Capsule on premature ovarian failure may be related to improving the level of differential metabolites in serum and restoring normal metabolic activities in rats.
