Study on Metabolomics of Stable Angina Pectoris of Coronary Artery Heart Disease with Spleen Deficiency and Phlegm Turbidity Syndrome Based on LC-MS
- VernacularTitle:基于液相色谱-质谱分析冠心病稳定型心绞痛脾虚痰浊证代谢组学研究
- Author:
Changbin YUAN
1
;
Yuzhu YAO
;
Yongming LIU
;
Meijia CHENG
;
Simeng XIE
;
Yetao JU
;
Shiyu ZHANG
;
Yifan ZENG
;
Huiyong ZHANG
;
Guanlin YANG
;
Dongyu MIN
Author Information
- Keywords: Stable angina pectoris of coronary artery heart disease; Spleen deficiency and phlegm turbidity syndrome; Metabonomics; Liquid chromatography-mass spectrometry
- From: World Science and Technology-Modernization of Traditional Chinese Medicine 2023;25(9):3105-3113
- CountryChina
- Language:Chinese
- Abstract: Objective To analyse the differential metabolites and related metabolic pathways in stable angina pectoris of coronary artery heart disease with spleen deficiency and phlegm turbidity syndrome by serum metabolomics.Methods This study observed 60 patients with stable angina pectoris of coronary artery heart disease with spleen deficiency and phlegm turbidity syndrome and 60 healthy volunteers in the same period.Liquid chromatography-mass spectrometry(LC-MS)was performed on the serum metabonomics.The differential metabolites were identified by multivariate statistical analysis of the original spectrogram and original data,and enrichment analysis of KEGG metabolic pathway was analyzed.Results A total of 60 patients in the group of stable angina pectoris of coronary artery heart disease with spleen deficiency and phlegm turbidity syndrome participated in the study,and a total of 60 healthy volunteers in the control group participated in the study.There was no statistical difference in general information and biochemical indicators between the two groups(P>0.05);Eighteen differential metabolites were found respectively,including phenylacetaldehyde,orthophosphate,guanosine,diethyl phosphate,2-dehydro-d-gluconate,guanine and 5-(2-hydroxyethyl)-4-methylthiazole down-regulated expression,taurocholate,2-propylglutaric acid,8-amino-7-oxononanoate,l-tyrosine,s-sulfo-l-cysteine,cyclohexanecarboxylic acid,porphobilinogen,(r)-acetoin,octanoylglucuronide,melatonin and solanine up-regulated expression,involving phenylalanine metabolism,thiamine metabolism,purine metabolism.Conclusion The differential metabolites reveal the metabolic essence of stable angina pectoris of coronary artery heart disease with spleen deficiency and phlegm turbidity syndrome from the micro level,and can provide clues for clinical early warning of patients with stable angina pectoris of coronary artery heart disease with spleen deficiency and phlegm turbidity syndromet.
