Protective Effect and Mechanism of Youning Bawei Powder on Dexamethasone Induced Non-alcoholic Fatty Liver Disease in Rats
- VernacularTitle:优宁八味散对地塞米松诱导大鼠非酒精性脂肪肝的保护作用及机制研究
- Author:
Hada WU
1
;
Shanhu CAO
;
Fang XIAO
;
Baoguang BAO
;
Bilige WENDUSU
Author Information
- Keywords: Mongolian Medicine Youning Bawei Powder; NAFLD; Disorder of lipid metabolism; CYP2E1; Reducing lipid and protecting liver
- From: World Science and Technology-Modernization of Traditional Chinese Medicine 2023;25(8):2785-2791
- CountryChina
- Language:Chinese
- Abstract: Objective To investigate the protective effect of Mongolian Medicine Youning Bawei Powder(MMYBP)on the liver of non-alcoholic fatty liver disease(NAFLD)model rats and its mechanism.Methods SD rats were randomly divided into normal group,model group,Yi Shan Fu group,MMYBP high and low dose groups.The normal group was fed with ordinary diet,and the other four groups were injected with dexamethasone and fed with high-fat diet,then establish NAFLD model.NAFLD model was treated with Yi Shan Fu,MMYBP high dose and low dose for one month.The liver index and pathological changes of liver tissue were detected.Serum alanine aminotransferase(ALT),aspartate aminotransferase(AST),triglyceride(TG)and liver triglyceride(TG),malondialdehyde(MDA),superoxide dismutase(SOD)and free fatty acid(FFA)indexes were measured by enzyme linked immunosorbent assay(ELISA).The expression level of CYP2E1 mRNA in liver tissue was measured by reverse transcription PCR(RT-PCR).The expression of cytochrome P4502E1(CYP2E1)protein in liver tissue was measured by Western blot.Results The liver index of MMYBP high dose group was significantly lower than that of model group(P<0.05).The contents of AST,ALT and TG in serum were significantly lower than those in model group(P<0.05).The contents of TG,FFA and MDA in liver tissue were significantly lower than those in model group(P<0.05).The pathological changes of liver were significantly improved compared with the model group.The expression levels of CYP2E1 protein and CYP2E1 mRNA in liver tissue were significantly lower than those in model group.Conclusion MMYBP can reduce the expression level of CYP2E1 protein and CYP2E1 mRNA related to lipid metabolism in NAFLD,improve liver function and lipid metabolism disorder,reduce lipid and protect liver in NAFLD,and delay the progression of NAFLD to liver fibrosis and cirrhosis.
