To Explore the Mechanism of Tonifying Kidney and Promoting Pharynx Prescription in the Treatment of IgA Nephropathy Based on TLR4 Signaling Pathway
10.11842/wst.20220917003
- VernacularTitle:基于TLR4信号通路探讨补肾利咽方治疗IgA肾病作用机制
- Author:
Di ZOU
1
;
Shoulin ZHANG
;
Hongbao ZHANG
;
Yinping WANG
Author Information
1. 长春中医药大学附属医院 长春 130021
- Keywords:
IgA nephropathy;
Tonifying kidney and promoting pharynx prescription;
Toll-like receptor 4;
Abnormal glycosylation of IgA1;
Model rats
- From:
World Science and Technology-Modernization of Traditional Chinese Medicine
2023;25(8):2759-2764
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of tonifying kidney and promoting pharynx prescription on IgA nephropathy model rats and the mechanism of regulating the TLR4 signaling pathway.Methods After SD rats were randomly divided into groups,the rat model of IgA nephropathy was replicated.After the medication,urinary RBC,UTP,blood creatinine,urea nitrogen,renal tissue TLR4,MyD88,NF-κB,MCP-1 expression,C1GALT1 and cosmoc mRNA expression in peripheral blood,TLR4,NF-κB and IL-6 in serum were observed.Results ①Compared with the blank group,urinary RBC,UTP,and renal tissue TLR4,MyD88 and NF-κB,MCP-1 expression,C1GALT1 and cosmoc mRNA expression in peripheral blood,TLR4,NF-κB and IL-6 in serum increased in the model group(P<0.05).②Compared with the model group,UTP,urinary RBC,renal tissue TLR4,MyD88 and NF-κB,MCP-1 expression,C1GALT1 and cosmoc mRNA expression in peripheral blood,the content of TLR4 and NF-κB,IL-6 were decreased(P<0.05)in tonifying kidney and promoting pharynx prescription group;There was no significant change in renal function.Conclusions ①The tonifying kidney and promoting pharynx prescription can effectively reduce hematuria and proteinuria in rats with IgA nephropathy.②The tonifying kidney and promoting pharynx prescription can reduce renal injury by improving the immune response mediated by TLR4 Signal transduction system induced by mucosal infection and reducing abnormal glycosylation IgA1 by adjusting key enzyme C1GalT1 and molecular companion Cosmc in IgA1 glycosylation process.
