Oleuropein Alleviates Gestational Diabetes in Mice Partly through Activating AMPK Signaling
10.11842/wst.20220608004
- VernacularTitle:橄榄苦苷通过激活AMPK信号通路缓解小鼠妊娠糖尿病
- Author:
Junfang CHEN
1
;
Xia WANG
;
Xia YAO
;
Jing LI
;
Aixiang CHEN
Author Information
1. 长治医学院附属和济医院 长治 046000
- Keywords:
Oleuropein;
Gestational diabetes mellitus;
Inflammation;
AMPK signaling
- From:
World Science and Technology-Modernization of Traditional Chinese Medicine
2023;25(7):2621-2627
- CountryChina
- Language:Chinese
-
Abstract:
Objective The objective of this study was to investigate the effects and mechanisms of plant-derived oleuropein on GDM mice.Methods Oleuropein was administered to GDM mice at the dose of 10 mg·kg-1·d-1.Body weight and blood glucose were recorded.The levels of interleukin-1β(IL-1β),interleukin-6(IL-6),tumor necrosis factor-α(TNF-α)and monocyte chemoattractant protein-1(MCP-1)were measured by ELISA and RT-qPCR to assess the inflammation level in GDM mice,the activation of Adenosine 5′-monophosphate-activated protein kinase signaling pathway was measured by Western blot.Gestational outcome was analyzed through comparing litter size and birth weight.Results Oleuropein attenuated the elevated body weight of GDM mice and efficiently reduced blood glucose,and restored insulin sensitivity.Oleuropein treatment also reduced oxidative stress and inflammation levels,activated AMPK signaling pathways in the liver tissues of GDM mice,and significantly improved gestational outcome in mice.Conclusion Oleuropein is effective in alleviating symptoms of GDM and improving gestational outcome in the mouse model.This effect is achieved by attenuating oxidative stress and inflammation,which is mediated by the AMPK signaling pathway.
