Identification of Four Diterpenoid Alkaloids in Radix Aconiti Kusnezoffii by SCX-SPE Combined with UHPLC-Q-Exactive Orbitrap MS
- VernacularTitle:SCX-SPE结合UHPLC-Q-Exactive Orbitrap MS分析草乌中的四类二萜生物碱
- Author:
Hongyan ZHOU
1
,
2
;
Jing XU
;
Yifang CUI
;
Shuyi SONG
;
Xianming LAN
;
Bing WANG
;
Jiayu ZHANG
Author Information
- Keywords: Radix aconiti kusnezoffii; Diterpene alkaloids; Strong acid cation exchange resin solid phase extraction column(SCX-SPE); Ultra high performance liquid chromatography-Q-Exactive Orbitrap mass spectrometry(UHPLC-Q-Exactive Orbitrap MS)
- From: World Science and Technology-Modernization of Traditional Chinese Medicine 2023;25(7):2574-2585
- CountryChina
- Language:Chinese
- Abstract: Objective To comprehensively characterize the diterpene alkaloids in Radix Aconiti Kusnezoffii.Methods The diterpene alkaloids were isolated and purified by strong acid cation exchange resin solid phase extraction column(SCX-SPE),and identified by ultra high performance liquid chromatography-Q-Exactive Orbitrap mass spectrometry(UHPLC-Q-Exactive Orbitrap MS).Results A total of 99 diterpene alkaloids were identified from Radix Aconiti Kusnezoffii,including 27 diester diterpene alkaloids(DDA),29 monoester diterpene alkaloids(MDA),40 amide diterpene alkaloids(ADA),2 polyester diterpene alkaloids(PDA)and 1 long-chain ester diterpene alkaloid(LDA).Conclusion The SCX-SPE combined with UHPLC-Q-Exactive Orbitrap MS method,established in this paper,can rapidly identify a large number of diterpene alkaloids in Radix Aconiti Kusnezoffii,which provides scientific proof for the study of pharmacodynamic substance basis and quality control of Radix Aconiti Kusnezoffii.
