The Effects of Rhubarb on Reproduction and Embryonic Development of Pregnant Rats Based on Amniotic Fluid Lipomics
- VernacularTitle:基于羊水脂质组学技术探索大黄对孕鼠生殖及胚胎发育影响
- Author:
Siying LI
1
;
Huafeng FANG
;
Ying ZHONG
;
Jianya XU
;
Jinjun SHAN
Author Information
- Keywords: Rhubarb; Rats; Reproductive toxicity; Embryonic development; Amniotic fluid lipidomics
- From: World Science and Technology-Modernization of Traditional Chinese Medicine 2023;25(6):1949-1960
- CountryChina
- Language:Chinese
- Abstract: Objective To observe the metabolomic changes of amniotic fluid in control group and administration group,and to explore the toxicity of aqueous extract of rhubarb on reproduction and embryonic development of pregnant rats.Methods Pregnant rats in teratogenic sensitive period were given rhubarb aqueous extract by gavage for 10 days.The toxicity of rhubarb to maternal rats and the abnormal conditions of dead fetus and absorbed fetus were observed.The amniotic fluid samples were detected by liquid chromatography-mass spectrometry(LC-MS),the amniotic fluid metabolic profiles were compared by principal component analysis(PCA)and partial least squares(OPLS-DA),and the differential lipids were analyzed by metaboanalyst 5.0.Results Rhubarb administration in the teratogenic sensitive period can significantly reduce the number of live fetuses,and lead to adverse phenomena such as absorption of fetuses and premature death of fetuses.The preliminary results of lipomics showed that rhubarb could cause the metabolic disorder of amniotic fluid in pregnant rats,and there were metabolic abnormalities in lipids in amniotic fluid such as PI,PC and LPC.Conclusion Under the equivalent dose of the maximum dose recommended by the clinic,the aqueous extract of rhubarb has certain reproductive and embryonic toxicity to rats;Rhubarb may affect the normal development of rat embryos by causing the disorder of lipid metabolism in amniotic fluid.
