The effects of cannabidiol on the expression of NLRP3 inflammasome following multiple cerebral concussions in rats
10.16557/j.cnki.1000-7547.2024.01.014
- VernacularTitle:大麻二酚对多重脑震荡大鼠NLRP3炎性小体表达的影响
- Author:
Chunze YU
1
,
2
;
Lifang YANG
;
Zhaoda DUAN
;
Yujia YANG
;
Chunyun WU
;
Jianyun YU
;
Li YANG
Author Information
1. 昆明医科大学基础医学院人体解剖与组织胚胎学系,昆明 650500
2. 本溪市中心医院麻醉科,本溪 117099
- Keywords:
cannabidiol(CBD);
multiple cerebral concussions(MCC);
NOD-like receptor pyrindomain-associat-ed protein 3(NLRP3)inflammasome;
inflammation
- From:
Chinese Journal of Neuroanatomy
2024;40(1):98-104
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effects of cannabidiol(CBD)on the NOD-like receptor protein 3(NLRP3)inflammasome in the brains of rats with multiple cerebral concussions(MCC).Methods:Rats were subjec-ted to the MCC model and divided into Sham,MCC,vehicle(MCC+TW),CBD-L(10 mg/kg),and CBD-H(40 mg/kg)groups.Immunofluorescence double staining was used to observe changes in NLRP3 and microglial cells in the brain,and Western Blot was performed to detect the expression changes of the NLRP3 inflammasome.Results:A sig-nificant increase in lectin-positive microglial cells of the cortex with enlarged cell bodies and elevated immunofluores-cence intensity of NLRP3 in the activated microglial cells was revealed by immunofluorescence double staining following MCC(P＜0.05).The immunofluorescence intensity of NLRP3 in the activated microglial cells was downregulated by the administration of CBD,with a more pronounced effect observed in the CBD-H group compared to the CBD-L group(P＜0.05).The expression of NLRP3,caspase-1,and apoptosis-associated speck-like protein(ASC)in the cortex,hippocampus,and basal ganglia of rats following MCC was significantly increased,as shown by Western Blot analysis(P＜0.05),and cortical areas are more elevated.The expression of these proteins in different brain regions was reduced by CBD-10 and CBD-40 intervention(P＜0.05).Conclusion:Cannabidiol can reduce the inflammatory response of multiple cerebral concussions rats through NLRP3 inflammasome and protect nerve tissue.
