The effects of minocycline on neuroinflammation in rats with post-traumatic stress disorder
10.16557/j.cnki.1000-7547.2024.01.010
- VernacularTitle:米诺环素对创伤后应激障碍大鼠神经炎症的影响
- Author:
Chong ZHANG
1
;
Zuolin SHI
;
Zhen WANG
;
Yushu DONG
Author Information
1. 中国人民解放军北部战区总医院神经外科,沈阳 110016
- Keywords:
minocycline;
post-traumatic stress disorder(PTSD);
neuroinflammation;
microglia;
hippocampus;
rat
- From:
Chinese Journal of Neuroanatomy
2024;40(1):73-77
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of minocycline on neuroinflammation of rats with post-traumatic stress disorder(PTSD).Methods:The rat model of PTSD was prepared by a single prolonged stress(SPS)method,and the rats were treated with minocycline(PTSD+Mino group)or normal saline(PTSD group)by gavage.The behavioral changes of rats were detected by light-dark box test.The expression of ionized calcium-binding adapter molecule 1(Iba-1)in hippocampus was detected by immunohistochemical staining.The contents of IL-1β and TNF-α in hippocampus were detected by ELISA,and the expression levels of IL-1β and TNF-α mRNA in hippocampus were detected by real-time RT-PCR(qRT-PCR).Results:After 3 days of SPS stimulation,the anxiety-like behavior of rats was obvious,the expression of Iba-1 in hippocampus was increased,and the contents of IL-1β and TNF-α in hippocampus were in-creased.Minocycline treatment significantly reduced anxiety-like behavior and decreased the expression of Iba-1 in the hippocampus of PTSD rats.Meanwhile,minocycline treatment also decreased the levels of IL-1β and TNF-α mRNA and protein in the hippocampus.Conclusion:Minocycline can improve the anxiety-like behavior of PTSD rats by inhibiting the activation of microglia.
