Mito-TEMPO inhibits the activation of BV2 microglia induced by lead exposure by reducing mitochondrial damage
10.16557/j.cnki.1000-7547.2024.01.009
- VernacularTitle:Mito-TEMPO通过减轻线粒体损伤抑制铅诱导的BV2小胶质细胞活化
- Author:
Chao CHEN
1
,
2
;
Zaihua ZHAO
;
Tao WANG
;
Jinsuo LU
;
Gang ZHENG
Author Information
1. 西安建筑科技大学环境与市政工程学院,西安 710055
2. 空军军医大学军事预防医学系军队劳动与环境卫生学教研室,特殊作业环境危害评估与防治教育部重点实验室,西安 710032
- Keywords:
Mito-TEMPO;
lead;
mitochondria;
inflammation;
BV2 microglia
- From:
Chinese Journal of Neuroanatomy
2024;40(1):65-72
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the mechanism of Mito-TEMPO inhibiting the activation of BV2 microglia induced by lead(Pb)exposure.Methods:Mouse microglia BV2 were cultured in vitro.The effects of different concentrations of lead exposure on the viability of BV2 cells were determined by MTT colorimetric method,and a model of lead expo-sure was developed and intervened with Mito-TEMPO antioxidant.Immunofluorescence staining was used to detect the expression of M1 activation marker CD86 protein.Enzyme-linked immunosorbent assay was used to detect the expression of pro-inflammatory factors interleukin-1 β(IL-1β),tumor necrosis factor α(TNF-α),interleukin-6(IL-6).Mito-chondrial superoxide indicator(MitoSOX)staining was used to detect the level of mitochondrial oxidative stress.JC-1 staining was used to detect mitochondrial membrane potential.The respiratory ability of mitochondria was detected by cell energy metabolism analysis system(O2k).Results:Compared with the control group,the expression of CD86 protein,the levels of pro-inflammatory cytokines IL-1β,TNF-α and IL-6 in BV2 cells increased significantly,the level of oxidative stress in mitochondria increased significantly,and the mitochondrial membrane potential and respiratory ability decreased significantly in lead exposed group.Mito-TEMPO treatment significantly reduced the oxidative stress and functional damage of mitochondria in BV2 cells,and significantly inhibited the M1 activation level of BV2 cells.Conclusion:The results show that Mito-TEMPO treatment can reverse the M1 activation of BV2 cells induced by lead exposure,and the specific mechanism may be related to the reduction of mitochondrial oxidative stress and dysfunction by Mito-TEMPO.
