The effects of gastrodin on TLR4 expression in microglia induced by ischemia and hypoxia
10.16557/j.cnki.1000-7547.2024.01.007
- VernacularTitle:天麻素对缺血缺氧诱导的小胶质细胞中TLR4表达的影响
- Author:
Shunda LI
1
;
Yongqiang ZHAO
;
Xueke YANG
;
Shuyu CHEN
;
Yan YANG
;
Yun YUAN
Author Information
1. 昆明医科大学基础医学院人体解剖与组织胚胎学系,昆明 650500
- Keywords:
hypoxia-ischemic brain damage(HIBD);
gastrodin(GAS);
toll-like receptor 4(TLR4);
oxygen glu-cose deprivation(OGD);
microglia;
rat
- From:
Chinese Journal of Neuroanatomy
2024;40(1):50-56
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To determine the effect of gastrodin(GAS)on toll-like receptor 4(TLR4)expression in mi-croglia after hypoxic-ischemic brain damage.Methods:Hypoxia-ischemic brain damage(HIBD)model was established in neonatal rat in vivo.Thirty 3 d SD rats of were randomly divided into there groups:Sham group,HIBD model group,HIBD model+gastrodin intervention group(HIBD+G).Oxygen glucose deprivation(OGD)model was established in BV2 cells in vitro,Control group(Control),oxygen glucose deprivation group(OGD),OGD+gastrodin intervention group(OGD+G)were randomly set in vitro.Both Western Blot and immunofluorescence staining techniques were used to detect the expression of TLR4 in cells of each group in vitro and in the left corpus callosum region in vivo.Results:The expression of TLR4 was significantly increased in OGD-induced microglia.After gastrodin intervention,TLR4 expression was decreased significantly(P＜0.05).Conclusion:GAS can inhibit the expression of TLR4 in activated microglia and thus play a neuroprotective role in HIBD.
