FOS expression in oxytocin and vasopressin positive neurons in paraventricular nucleus of mice induced by diabetes
10.16557/j.cnki.1000-7547.2024.01.005
- VernacularTitle:糖尿病诱导的小鼠室旁核催产素和加压素阳性神经元FOS表达
- Author:
Shumin WANG
1
,
2
;
Peng JIA
;
Shuting REN
;
Siting LYU
;
Ting ZHANG
;
Yanling YANG
;
Juan SHI
Author Information
1. 延安大学医学院生理学教研室,延安 716000
2. 空军军医大学基础医学院人体解剖与组织胚胎学教研室,西安 710032
- Keywords:
diabetic neuropathic pain(DNP);
hypothalamic paraventricular nucleus(PVN);
oxytocin(OXT);
vasopressin(VP);
mouse
- From:
Chinese Journal of Neuroanatomy
2024;40(1):35-42
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the feature of FOS expression in oxytocin-and vasopressin-positive neurons in the hypothalamic paraventricular nucleus(PVN)under different status of diabetes mellitus(DM).Methods:Intraperito-neal injection of vehicle or STZ in mice was conducted to establish control or diabetes model.Mechanical sensitivity was evaluated by von Frey filament tests to distinguish diabetic neuropathic pain(DNP)from without-pain group(DWP).The expression of FOS,oxytocin(OXT)-and vasopressin(VP)-positive neurons,as well as their double labeling was detected by immunohistochemical and immunofluorescent staining.Cell counting and comparison were made in groups.Results:FOS expression was easily detected in the PVN in the three groups(Control group,DNP group and DWP group)at 7 days,while that in DWP and DNP groups at 28 days was hardly detectable,with the number being signifi-cantly different from the 7 days group(P＜0.05 or 0.001).Likewise,compared with the control group,immunofluo-rescent signals for VP and OXT staining in the DNP and DWP groups also showed a trend of weakening as the modeling time increased(P＜0.05).The cell counting after double staining for VP or OXT with FOS showed that,in the DWP group at 7 days,the number of VP and FOS double-labeled neurons was 74.33±22.10,accounting for(56.64± 7.52)％of VP-positive cells,whereas the double labeling rate for OXT and FOS was only(10.44±3.14)％.In the DNP group at 7 days,the number of OXT and FOS double-labeled neurons was 51.00±31.80,accounting for(18.50 ±9.51)％of OXT-positive neurons,whereas the double labeling rate for VP and FOS was only(9.34±3.27)％.In contrast to these changes in 7 days group,the expression of FOS decreased sharply in the group of 28 days,thereby al-most no double-labeled neurons.Conclusion:The plasticity changes of oxytocin-and vasopressin-positive neurons in the PVN are different depending on the status of pain and non-pain,and the stage of disease progression.Understanding the changes is of great significance for unravelling the neural mechanism of diabetes and its complications.
