Isolation and identification of primary brain microvascular endothelial cells of rat using thin-layer cell culture method
10.16557/j.cnki.1000-7547.2023.06.014
- VernacularTitle:薄层液面培养法提取大鼠原代脑微血管内皮细胞及对其的鉴定
- Author:
Bolin LI
1
;
Fan ZHANG
;
Haiqin LIU
;
Yuanyu TANG
Author Information
1. 福建中医药大学中医学院,福州 350122
- Keywords:
thin-layer cell culture method;
brain microvascular endothelial cells(BMECs);
factor Ⅷ related an-tigen(FⅧRag);
rat
- From:
Chinese Journal of Neuroanatomy
2023;39(6):709-713
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To extract rat brain microvascular endothelial cells(BMECs)using thin-layer cell culture method.Methods:The brain cortex of four-week-old SD rats was obtained,which was chopped,sieved,and digested with type II collagenase to obtain microvascular segments.The amount of culture medium was strictly controlled,and it was inoculated into culture flasks for primary culture.The target cells were morphologically observed by using an invert-ed phase contrast microscope,and the factor Ⅷ related antigen(FⅧRag)was identified by using immunocytochemi-cal staining.Results:The target cells cultured in the inverted phase contrast microscope showed typical endothelial-like monolayer cobble stone-like mosaic growth;FⅧRag positive cells accounted for more than 99％of the total cells.Conclusion:Thin-layer cell culture method can successfully isolate and cultivate high-purity rat BMECs.
