Anthocyanins(C3G)alleviate epileptic seizures through antioxidant inhibition autophagy in the rat
10.16557/j.cnki.1000-7547.2023.06.012
- VernacularTitle:花青素(C3G)通过抗氧化抑制自噬缓解大鼠痫样发作
- Author:
Zi WANG
1
;
Rui NING
;
Xiangwei ZHANG
;
Shuhua WU
;
Ke GUO
;
Chong GUO
;
Jianmin LI
Author Information
1. 滨州医学院附属医院神经外科,滨州 256603
- Keywords:
epilepsy;
anthocyanins(C3G);
oxidative stress;
autophagy;
action potential;
rat
- From:
Chinese Journal of Neuroanatomy
2023;39(6):696-704
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the feasibility of anthocyanins(C3G)antioxidant inhibition of autophagy to al-leviate epilepsy.Methods:Seventy-five SD rats were randomly divided into 5 groups:Control group,pentetrazole(PTZ)group,hydrogen peroxide(H2O2)intervention group,3-methyladenine(3-MA)intervention group,and C3G intervention group.The seizure grade,latency,and frequency were documented.Electroencephalography was employed to detect abnormal electrical discharges in the brain across.Patch clamp technique was utilized to measure action poten-tials in hippocampal neurons for each group.The concentration of 4-hydroxynonenoic(4-HNE)hippocampus was deter-mined using a specific kit.Ultrastructural alterations in hippocampal neurons were examined through electron microsco-pyissl staining was performed to assess neuronal damage within the hippocampus.Immunohistochemical staining and Western Blot were conducted to evaluate expression levels of 15-LOX,GPX4,and LC3 proteins within the hippocampus of rats.Results:Compared with the control group,the PTZ group was completely ignited and the modeling was success-ful.Compared with other epilepsy groups,the seizure grade of C3G group decreased,abnormal discharge decreased,latency increased,hippocampal neuron excitability decreased,nishi content increased,4-HNE content,15-LOX expression and LC3Ⅱ/LC3Ⅰ ratio decreased,but GPX4 expression increased(P＜0.05).Conclusion:The oxidative stress induced by epilepsy can induce excessive autophagy of neurons,and C3G can alleviate the occurrence and devel-opment of epilepsy by anti-oxidation and inhibition of autophagy.
