Morphological study on abnormal brain development induced by hypoxia in neonatal mice
10.16557/j.cnki.1000-7547.2023.06.003
- VernacularTitle:缺氧致新生小鼠脑发育异常的形态学研究
- Author:
Jie YUAN
1
,
2
;
Yingqi LIU
;
Yuhao DONG
;
Yiyuan HUANG
;
Sufang JIANG
;
Ming ZHANG
;
Xianghui ZHAO
Author Information
1. 西北大学生命科学学院,西安 710069
2. 空军军医大学基础医学院神经生物学教研室,西安 710032
- Keywords:
hypoxia;
anterior cingulate cortex(ACC);
oligodendrocyte(OL);
myelin;
interneuron;
mouse
- From:
Chinese Journal of Neuroanatomy
2023;39(6):633-640
- CountryChina
- Language:Chinese
-
Abstract:
Objective:Neonatal mice hypoxia model was established to observe the responses of the main neural cell types in cognition-related brain areas.Methods:Pups at postnatal day 2(P2)were subjected to 10％oxygen for suc-ceeding 5 days,and harvested at different development stage for histologic study.Immunofluorescence histochemistry was used to compare the changes of oligodendrocyte density,mature oligodendrocyte ratio and myelin protein level in corpus callosum(CC)and motor cortex(M1)after hypoxia,as well as the expression changes of excitatory and inhibi-tory neurons in anterior cingulate cortex(ACC),hippocampus(Hippo)and sensory cortex(S1).Furthermore,the density changes of different types of inhibitory intermediate neurons,microglia and astrocytes in ACC were compared.At the same time,the effect of hypoxia on the expression of synaptic proteins was also detected.Results:Quantitative immunofluorescence results showed lower myelin protein levels and mature oligodendrocyte ratio in CC and M1 of hypoxic mice compared with control mice.There was no significant difference in the number of excitatory neurons in ACC,but the number of gamma-aminobutyric acid(GABA)neurons in ACC,Hippo,and S1 were significantly reduced,especially parvalbumin neuron,ssomatostatin neurons,and vasoactive intestinal polypeptide neurons in ACC.The number of excitatory synapses labeled by vesicular glutamate transporter 1(VGluT1)and inhibitory synapses labeled by gephyrin were significantly decreased in ACC of hypoxic mice.Although there was no significant difference in astrocyte and microglia numbers,microglia were activated after hypoxic injury.Conclusion:Chronic hypoxia will lead to changes in the development of oligodendrocytes and interneurons,impair synapse formation.These results provide an important experimental basis for exploring the neural mechanism of diseases related to abnormal brain intelligence devel-opment.
