Effects of CEP55 on the proliferation ability and H3K9 trimethylation of bladder cancer cells
10.3760/cma.j.cn.115807-20230603-00161
- VernacularTitle:CEP55通过H3K9三甲基化对膀胱癌细胞增殖能力的影响
- Author:
Yisheng GAO
1
;
Chengyun ZHANG
;
Lei GAO
;
Fengyuan CHE
;
Yinghao ZHANG
;
Tong LU
;
Zhiming WANG
;
Jie LIU
Author Information
1. 临沂市人民医院泌尿外科，临沂 276000
- Keywords:
Bladder cancer;
Centrosome protein 55;
H3K9;
Methylation
- From:
Chinese Journal of Endocrine Surgery
2023;17(6):716-719
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of centrosome protein 55 (CEP55) on the proliferation of bladder cancer cells and its related molecular mechanism.Methods:Western blot was used to detect the expression of CEP55 and H3K9me3 in normal bladder tissue cells (SV-HUC-1) and bladder cancer cells (T24) . The bladder cancer cells T24 were divided into experimental group and control group. The experimental group cells were transfected with siRNA-CEP55, and the control group cells were transfected with siRNA-MOCK. The expression levels of CEP55, H3K9 and H3K9me3 in each group of cells were detected by Western blot. The proliferation ability of each group of cells was detected by CCK8 assay.Results:Western blot assay showed that the expression of CEP55 and H3K9me3 in T24 cells was 0.83±0.15 and 1.01±0.19 respectively. The expressions of CEP55 and H3K9me3 in bladder epithelial SV-HUC-1 cells were 0.35±0.09 and 0.44±0.10 respectively. The expressions of CEP55 and H3K9me3 in bladder cancer cells were higher than those in normal bladder cells (all P<0.05) . siRNA-CEP55 successfully reduced the expression of T24 CEP55 in bladder cancer cells. The absorbance of T24 cells in the experimental group was 1.109±0.105, which was significantly lower than that in the control group (2.208±0.104) . Low expression of CEP55 reduced the proliferation ability of T24 cells ( P<0.05) . Western blot results showed that H3K9 was not significantly changed in T24 cells in the experimental group, and H3K9me3 expression decreased significantly ( P<0.05) . Conclusion:CEP55 can inhibit the proliferation of bladder cancer cells by reducing the trimethylation of H3K9.
