Inhibition effect of temozolomide on proliferation of human glioma cells by autophagy-induced pyroptosis
10.3760/cma.j.cn.115807-20221221-00380
- VernacularTitle:替莫唑胺通过自噬诱导焦亡抑制人脑胶质瘤细胞增殖作用的研究
- Author:
Shiwen LYU
1
;
Zhun HUANG
;
Yalan ZHU
;
Chunxia HONG
Author Information
1. 浙江大学医学院附属金华医院药学部，金华 321000
- Keywords:
Temozolomide;
Glioma cells;
Autophagy;
Pyroptosis
- From:
Chinese Journal of Endocrine Surgery
2023;17(5):624-629
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of temozolomide on autophagy of human glioma cells, and the inhibition of autophagy induced pyrocytosis on the proliferation of human glioma cells.Methods:2-64 μ mol/L of temozolomide was used to treat glioma U251 cells cultured in vitro. MTT assay was used to detect cell viability, MDC staining was used to detect autophagic vesicles in cells, cloning assay was used to detect cell proliferation, RT qPCR was used to detect the expression level of pyroptosis related mRNA in cells, Western blot was used to detect the expression of autophagy related proteins and pyroptosis related proteins in cells, and the relationship between autophagy and pyroptosis was detected by adding autophagy inhibitors.Results:Temozolamide could induce autophagy of human glioma cells, and significantly induce tumor cells to pyroptosis, thereby inhibiting the proliferation of tumor cells; RT qPCR results showed that caspase-1, GSDMD, IL-1 after temozolomide administration compared with the normal group β, the mRNA expression levels of IL-18 and NLRP3 increased significantly; Western blot results showed that Cleaved-caspase-1, Cleaved-N-terminalGSDMD, IL-1 β、IL-18 and NLRP3 protein were up-regulated; The incidence of pyroptosis decreased after the addition of autophagy inhibitors.Conclusion:Temozolamide can induce autophagy of human glioma cells, and then lead to pyroptosis, which plays an inhibitory role in proliferation.
