Anticancer effect of bleomycin combined with cisplatin on human gastric cancer cells in vitro
10.3760/cma.j.cn.115807-20220725-00201
- VernacularTitle:博来霉素联合顺铂对人胃癌细胞的体外抗癌作用研究
- Author:
Zi YANG
1
;
Yonghui CUI
;
Zhihan LI
;
Zhiheng ZHENG
;
Xiliang ZHANG
;
Huijie GAO
Author Information
1. 河南省商丘市第一人民医院消化内科，商丘 476000
- Keywords:
Bleomycin;
Cisplatin;
Gastric cancer;
Reactive oxygen species;
JAK2/STAT3 pathway
- From:
Chinese Journal of Endocrine Surgery
2023;17(4):474-479
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the in vitro anticancer effect of bleomycin (Ble) combined with cisplatin (DDP) on human gastric cancer cells.Methods:CCK-8 experiment was conducted to detect the effect of Ble and DDP alone or in combination on the proliferation of gastric cancer cells; Flow cytometry was used to detect changes in cell apoptosis and changes in reactive oxygen species. Cell scratch test was employed to detect cell migration; Western blot experiment was conducted to detect protein expression.Results:The results of CCK-8 showed that 20 μM DDP could inhibit the proliferation of six types of gastric cancer cells by 40%, while the effect of 20 μM Ble was only about 20%. When used in combination, the inhibitory rate on six types of gastric cancer could reach over 60%, with the strongest inhibitory effect on AGS cells. When 10 μM Ble and 10 μM DDP were combined, the proportion of AGS apoptotic cells reached 75.68%, higher than that of the two drugs treated with 20%, respectively 20 μM processing effect. When 10 μM Ble and 10 μM DDP were used in combination, the number of migrating and invading cells was significantly reduced, and the inhibition rate reached 50%. Compared with single use, the difference was statistically significant ( P<0.05). Flow cytometry results showed that the combination of Ble and DDP promoted cancer cell apoptosis by up-regulating the level of reactive oxygen species in cells; Western blot results showed that the expression of p-JAK2 and p-STAT3 decreased after Ble and DDP were combined, indicating that the JAK2/STAT3 signal pathway was inhibited. Conclusions:The combination of Ble and DDP can inhibit the proliferation and migration of gastric cancer cells, promote the apoptosis of gastric cancer cells, and play a synergistic anti-cancer effect. Its mechanism may be related to the up-regulation of ROS levels in cells, thereby inhibiting the activation of the JAK2/STAT3 pathway.
