Progresses of phage display technology application in fully human antibody discovery
10.13431/j.cnki.immunol.j.20230118
- VernacularTitle:噬菌体展示技术在全人源性抗体发现中的应用
- Author:
Bixia LIU
1
;
Yuan LIU
;
Jing XIE
;
Zhengbin GUO
;
Bin WANG
;
Qianfei ZUO
;
Rui ZHANG
Author Information
1. 610031 成都,西南交通大学医学院
- Keywords:
Phage antibody display technology;
Fully humanized antibodies;
Antibody library;
Antibody screening
- From:
Immunological Journal
2023;39(10):910-915
- CountryChina
- Language:Chinese
-
Abstract:
Phage antibody display technology is currently the most widely used in vitro antibody screening technology,which uses bacteriophages as a vector,and inserts exogenous antibody library genes into phage capsid protein genes,and expresses the capsid protein on the phage surface while also displays the antibody protein.Antibody drugs play an important role in tumor immunity and microbial immunity due to their targeting advantages,which is also an important driving force for them to become a hot spot in the field of pharmaceutical research and development.Therefore,this article reviews the background,basic principles,antibody library types and antibody fragment types of phage display technology,and looks forward to the latest progress and application prospects of fully human antibodies.
