Application value of fumarate hydratase and 2-succinocysteine in primary screening of fumarate hydratase-deficient uterine leiomyoma
10.13315/j.cnki.cjcep.2024.03.006
- VernacularTitle:延胡索酸水合酶与2琥珀酸半胱氨酸在初筛延胡索酸水合酶缺陷型子宫平滑肌瘤的应用价值
- Author:
Tiantian FANG
1
;
Feng SHI
;
Ning QIAN
;
Weiju ZHANG
;
Yun GU
Author Information
1. 南京医科大学附属妇产医院(南京市妇幼保健院)病理科,南京 210004
- Keywords:
FH deficient leiomyoma;
leiomyoma with bizarre nuclei;
fumarate hydratase;
2-succinocysteine
- From:
Chinese Journal of Clinical and Experimental Pathology
2024;40(3):251-254
- CountryChina
- Language:Chinese
-
Abstract:
Purpose To investigate the expression of fuma-rate hydratase(FH)and 2-succinocysteine(2SC)in leiomyoma with singular nuclear features,and to analyze the diagnostic val-ue of these two proteins in fumarase hydratase-deficient(FH-d)uterine leiomyomas.Methods Sixty-seven cases of leiomyoma with singular nuclear features were collected.The expression was detected by immunohistochemical EnVision method,and the relationship between the expression of FH,2SC and the morpho-logical characteristics of FH-d uterine leiomyomas was analyzed,and the relevant literature was reviewed.Results There were 67 patients of leiomyoma with singular nuclear features,among which 27 had FH-d leiomyoma.The positive rate of FH was(2/27,7.41％),significantly lower than that of leiomyomas without FH-d(31/40,77.50％).The positive rate of 2SC(26/27,96.30％)was significantly higher than that of leiomyo-mas without FH-d(11/40,27.50％).The statistical results showed that there was a correlation between the expression of FH and 2SC(P＜0.05);positive expression of FH and 2SC was associated with significant eosinophilic nucleoli,perinucleolar vacuoles,eosinophilic inclusions and antler vessels(P＜0.05).The sensitivity and specificity of FH immunohistochemi-cal identification of FH defective leiomyoma were 92.59％and 77.50％,respectively.The sensitivity and specificity of 2SC immunohistochemical were 96.30％and 72.50％for FH defec-tive leiomyomas,respectively.Conclusion In most leiomyomas with FH-d morphology,FH expression is negative and 2SC ex-pression is positive.The combined detection of FH and 2SC can significantly improve the diagnosis of FH defective leiomyoma.
