Immune microenvironment and clinicopathological features in brain metastases of non-small cell lung cancer
10.13315/j.cnki.cjcep.2023.11.007
- VernacularTitle:非小细胞肺癌脑转移免疫微环境、临床病理特征分析
- Author:
Chang WAN
1
;
Jingdan PANG
;
Zhengsheng WU
;
Bin WANG
;
Jing XUE
;
Yingying DU
Author Information
1. 安徽医科大学第一附属医院肿瘤科,合肥 230022
- Keywords:
lung neoplasm;
non-small cell lung cancer;
tumor microenvironment;
brain metastases
- From:
Chinese Journal of Clinical and Experimental Pathology
2023;39(11):1316-1321
- CountryChina
- Language:Chinese
-
Abstract:
Purpose To investigate the expression of PD-1,PD-L1,CD3 and CD8 in the immune microenvironment of non-small cell lung cancer(NSCLC)and their clinical signifi-cance.Methods The clinical data of 39 patients with NSCLC brain metastasis(BM)were collected.The expression of PD-1,PD-L1,CD3 and CD8 in the tumor and stroma of BM was detec-ted using an immunofluorescence-based tissue microenvironment analysis panel.Targeted sequencing was carried out to catalog cancer-related genes.The clinical pathological features were an-alyzed with review of relevant literature.Results Thity-nine patients with NSCLC presented with tumor-infiltrating lympho-cytes(TIL)in different degree.CD3+TIL(P=0.000 7)and CD8+TIL(P=0.0006)were more prominent in the tumor stroma,and the positive PD-L1 expression was significantly higher in the interstitial tissues of tumor(P=0.025 8).Com-pared with the whole wild-type driver gene cohort,the expres-sion of PD-L1 in the stroma of BM was significantly increased in the EGFR mutation cohort(P=0.039).Patients with high in-filtration of stroma CD8+TIL had longer median overall survival than those with low infiltration(16 months vs 6 months,P=0.032);PD-L1-positive patients(36 months)were longer sur-vival time than PD-L1-negative patients(5.5 months,P=0.056).Compared with lung primary lesions,the variant allele frequencies(VAFs)in BM generally increased,and samples with higher VAFs corresponded to higher expression of PD-1,PD-L1,CD3 and CD8.Conclusion The TIL infiltration is most prominent in the stroma of NSCLC BM.The EGFR muta-tion of a tumor might affect the immune microenvironment of me-tastases;PD-L1 expression and TIL infiltration were correlated with overall survival.
