Significance of BRAFV600E protein and gene detection in benign,malignant and borderline thyroid diseases
10.3969/j.issn.1005-6483.2023.12.020
- VernacularTitle:BRAFV600E在甲状腺良恶性及交界性病变检测中的价值
- Author:
Jianxing JI
1
;
Qinnuan SUN
Author Information
1. 010000 呼和浩特,内蒙古医科大学基础医学院
- Keywords:
BRAFV600E;
papillary thyroid carcinoma;
borderline lesion;
immunohisto-chemistry
- From:
Journal of Clinical Surgery
2023;31(12):1180-1183
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the significance of BRAFV600Ein the detection of benign,malignant and borderline thyroid lesions.Methods A total of 273 formalin fixed and paraffin-embedded specimens of papillary thyroid carcinoma(PTC)were selected for study from January 2010 to December 2018.There were 254 cases of classical variant of papillary carcinoma(CVPTC)and 19 cases of follicular variant of papillary carcinoma(FVPTC).30 benign lesions were made into tissue microarray(TMA),and 17 uncertain borderline lesions diagnosed by HE.Polymerase chain reaction(PCR)was used to detect 17 cases of CVPTC 12 cases of FVPTC 17 cases of borderline lesions and 13 cases of benign lesions.BRAFV600E gene mutation and protein expression were observed.Results The positive expression rates of BRAFV600E protein in CVPTC,FVPTC,borderline lesion and benign lesion were 86.6％,15.9％,23.5％and 26.7％,respectively.Mutation rates of BRAFV600E gene in CVPTC,FVPTC,borderline lesions and benign lesions were 82.4％,41.7％,23.5％and 0,respectively.There were significant differences in expression of BRAFV600E protein in CVPTC,FVPTC,borderline lesions and benign lesions(P＜0.05).There was statistically significant difference between BRAFV600E gene mutation in CVPTC and FVPTC and borderline diseases(P＜0.05).The coincidence coefficient between BRAFV600E gene mutation and protein expression in CVPTC was K=0.821,P＜0.05.It was statistically significant.The positive expression of BRAFV600E protein in PTC was not correlated with gender,age,tumor size and lymph node metastasis(P＞0.05),but was correlated with tissue type and capsule invasion(P＜0.05).Conclusion BRAFV6001 has reference value in the diagnosis and differential diagnosis of benign and malignant thyroid and borderline thyroid lesions.
