Application of seminal carnitine assay in male fertility assessment and therapeutic monitoring of asthenospermia
10.13602/j.cnki.jcls.2024.02.06
- VernacularTitle:精浆肉碱测定在男性生育力评估和弱精子症疗效监测中的应用
- Author:
Jun KAI
1
;
Laiqing ZHU
;
Minhuan LI
;
Guohai SUN
;
Xue ZHOU
;
Xun WANG
;
Youfeng HAN
;
Liang SHI
Author Information
1. 南京大学医学院附属鼓楼医院男科,南京 210008
- Keywords:
asthenospermia;
seminal L-carnitine;
percentage of progressively motile sperm;
oral L-carnitine solution
- From:
Chinese Journal of Clinical Laboratory Science
2024;42(2):107-110
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compare seminal carnitine levels between normal males and asthenozoospermic patients,evaluate its correla-tion with progressive motility(PR)of sperm,and observe the effects of exogenous carnitine supplementation on asthenozoospermic pa-tients.Methods Semen samples were collected from 511 normal fertile males and asthenozoospermic patients.Seminal was measured using a fixed-time assay kit and the levels of carnitine were compared between the two groups.The consistency between seminal carni-tine and PR was assessed.Additionally,77 asthenozoospermic patients received L-carnitine(1 g/time,3 times/day,30 days/course).The levels of seminal carnitine and PR alteration pre-and post-treatment were monitored.Results The seminal L-carnitine level in the patients with asthenospermia([194.34±65.41]μmol/L)was significantly lower than that in normal fertile males([405.43±72.12]μmol/L)(P＜0.01).When the seminal L-carnitine level ≥325 μmol/L was set as the threshold,the statistical results showed that Kappa value was 0.81,with a diagnostic coincidence rate of 93.74％.After one course of administration of L-carnitine,the concentra-tion of seminal L-carnitine([356.03±84.87]μmol/L)and PR([32.69±8.35]％)were significantly higher those that before treat-ment([183.61±79.54]μmol/L and[16.56±7.74]％,P＜0.01).Conclusion The seminal carnitine assay kit could be used for ac-curate and high-throughput quantification of clinical samples,facilitating asthenozoospermia diagnosis and therapeutic efficacy evalua-tion.Exogenous carnitine supplementation may elevate seminal carnitine levels and sperm motility in asthenozoospermic patients and po-tentially improve their fertility.
