Improvement and application of culture method for hemorrhagic amniotic fluid cells
10.13602/j.cnki.jcls.2024.02.03
- VernacularTitle:血性羊水细胞培养方法的改良与应用
- Author:
Daoqi HUANG
1
;
Huayu TU
;
Keting TONG
;
Chaohong WANG
;
Jiansheng ZHU
Author Information
1. 安徽医科大学附属安徽省妇幼保健院遗传医学中心,合肥 230011
- Keywords:
hemorrhagic amniotic fluid;
combined culture method;
in situ culture method;
karyotype analysis
- From:
Chinese Journal of Clinical Laboratory Science
2024;42(2):90-92
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compare the success rates of different culture methods for hemorrhagic amniotic fluid.Methods Thirty-one hemorrhagic amniotic fluid samples from pregnant women who were subjected to chromosomal examination at Anhui Maternal and Child Health Hospital were collected from January 2021 to December 2022.Two culture methods,the slide in situ culture box method(re-ferred to as the slide method)and the plastic bottle culture combined with slide in situ culture box method(referred to as the combined culture method),were used for cell cultivation.All the cells were harvested and stained with Giemsa staining,then the number of eligi-ble karyotype was counted and the success rates were compared between the two methods.Results Among the 31 cases of the slide method,21 were successfully cultured with a success rate of 67.7％.For the combined culture method,all the 31 cases were success-fully cultured with a success rate of 100％.The success rate of the combined culture method was significantly higher than that of the slide method(P＜0.05).Of 31 bloody amniotic fluid samples,three cases were fresh bleeding,and an average number of eligible kary-otype was 8 in the slide method and 32 in the combined culture method.Twenty-eight cases were old bleeding,and an average number of eligible karyotype was 13 in the slide method and 53 in the combined culture method.The number of eligible karyotype in the com-bined culture method was significantly higher than that of the slide method(P＜0.05).Conclusion The combined culture method is suitable for the cultivation of hemorrhagic amniotic fluid samples,and should be worthy of promotion in the clinics.
