Screening of miRNA biomarkers in serum exosomes of patients with thyroid nodules at different iodine levels
10.13602/j.cnki.jcls.2024.01.14
- VernacularTitle:不同碘水平下甲状腺结节患者血清外泌体中miRNA生物学标志物的筛选
- Author:
Wenyuan CAO
1
;
Hongjian ZHAO
;
Hao XING
;
Hui ZHANG
;
Wei KONG
;
Qinghua LIU
;
Fengyan YIN
;
Qian HE
;
Weijia XING
Author Information
1. 山东第一医科大学公共卫生与健康管理学院,研究生院,济南 250000
- Keywords:
thyroid nodule;
iodine;
exosome;
microRNA
- From:
Chinese Journal of Clinical Laboratory Science
2024;42(1):62-66
- CountryChina
- Language:Chinese
-
Abstract:
Objective To comapre and analyze the differences and commonalities of expression profiles of serum exosomal microRNA between patients with thyroid nodules and healthy persons at different iodine levels,and then provide evidence for screening early diag-nostic markers of thyroid nodules at different iodine levels.Methods The peripheral blood samples from 10 patients with thyroid nod-ules and healthy volunteers at different iodine levels were collected.Their serum iodine levels were measured by the arsenic cerium cat-alytic spectrophotometry.Serum exosomal microRNA were extracted and the expression levels of microRNA were determined by the high-throughput sequencing technology.The differential target genes were predicted and further performed Gene ontology(GO)analysis and Kyoto Encyclopedia of Genes and Genomes(KEGG)analysis.Results Compared with healthy volunteers,there were 6 downreg-ulated miRNAs in the patients with thyroid nodules at different iodine levels,namely miR-324-5p,miR-6511b-3p,miR-9903,miR-550a-3p,miR-5001-3p,and miR-3688-3p.Differentially expressed exosomal microRNA could regulate the MAPK signaling path-way,PI3K-AKT signaling pathway,VEGF signaling pathway,and NF-κB signaling pathway.Conclusion Six differentially expressed microRNAs is identified,which may serve as biological markers for the early diagnosis of thyroid nodules at different iodine levels.
