Effect of calcium and vitamin D supplementation on bone formation around titanium implant in osteoporosis-induced rats.
- Author:
Jae Yeol LEE
1
;
Seok Young JEONG
;
Sang Hun SHIN
;
Gyoo Cheon KIM
;
Yong Deok KIM
;
In Kyo CHUNG
;
Uk Kyu KIM
Author Information
1. Department of Oral and Maxillofacial Surgery, School of Dentistry, Pusan National University, Busan, Korea. ssh8080@pusan.ac.kr
- Publication Type:Original Article
- Keywords:
Osteoporosis;
Implant;
Calcium;
Vitamin D
- MeSH:
Animals;
Bone Density;
Bone Marrow;
Calcium;
Collagen Type I;
Fibronectins;
Immunohistochemistry;
Models, Animal;
Osseointegration;
Osteogenesis;
Osteoporosis;
Rats;
Tibia;
Titanium;
Vitamin D;
Vitamins
- From:Journal of the Korean Association of Oral and Maxillofacial Surgeons
2008;34(3):276-284
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: The purpose of this study was to observe the effect of calcium and vitamin D to the titanium implant osseointegration in the osteoporosis-induced animal model. MATERIAL AND METHOD: Thirty-two rats, 10 weeks of age, were divided into two groups: experimental group was ingested additional calcium and vitamin D, and a control group was not. Titanium screw implant(diameter, 2.0 mm; length, 3.5 mm; pitch-height 0.4 mm) were placed into tibia of 32 rats, 16 in the control group and 16 in the experimental group. The rats were sacrificed at 1, 2, 4 and 8 weeks after implantation for histopathologic examination, histomorphometric analysis and immunohistochemistry with fibronectin and collagen type I antibody. RESULT: In histopathological findings, newly formed bone was seen at 2 weeks and became lamellar bone at 4 weeks, and mature trabecullar bone was seen at 8 weeks in experimental group. In control group, thickness of regenerated bone increased till 4 weeks gradually and trabecullar bone was seen at 8 weeks. In histomorphometric analysis, marrow bone density increased significantly in experimental group compared to control group. Fibronectin immunoreactivity was strong at 2 weeks in experimental group and reduced after 4 weeks gradually. But it was maintained continuously from 2 to 8 weeks in control group. Collagen type I immunoreactivity was very strong from 2 to 4 week in experimental group. And the amount of Collagen type I expression was more abundant in experimental group. CONCLUSION: The results of this study suggest that calcium and vitamin D supplementation promote bone healing around titanium implants in osteoporosis induced animals.
