Study on the Relationship Between OAT1 Expression and Renal Osteodystrophy in Rats with Chronic Renal Failure
10.12259/j.issn.2095-610X.S20240205
- VernacularTitle:慢性肾衰竭大鼠OAT1的表达对骨代谢的影响
- Author:
Wenjuan SHEN
1
;
Wenxin LIANG
;
Qing LI
;
Hongfang XU
;
Weiqin ZHANG
;
Haixin LI
Author Information
1. 昆明医科大学海源学院病理生理学教研室,昆明 云南 651700
- Keywords:
Organic anion transporter;
Chronic renal failure;
Osteodystrophy
- From:
Journal of Kunming Medical University
2024;45(2):32-38
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the changes of OAT expression in bone cells in chronic renal failure(CRF)and involved mechanism,and to explore the effect of OAT expression on bone metabolism.Methods Ra-ndomly divide the rats into a control group(n=6)and a model group(n=6).The model group established a rat chronic renal failure model using"single nephrectomy+adenine gavage"method,and the red blood cell(RBC)and hemoglobin(HGB)of the rat body were measured using a blood routine analyzer;Measure indicators such as creatinine(Cr),urea nitrogen(BUN),uric acid(UA),blood calcium(Ca2+),and blood phosphorus(P3+)using a fully automated biochemical analyzer;Pathological examination of rat kidneys;X-ray examination of rat tibia;Immunohistochemical examination of bone tissue OAT1 level.Results The bone density of the model group rats is lower than that of the control group;The calcium and phosphorus metabolism of rats in the model group was in metabolic disorder,and the OAT1 value of bone tissue binding was much lower than that of the control group(P= 0.0018),which was statistically significant.(P=0.0018)Conclusion Chronic renal failure affected the binding ability of OAT1 in bone tissue,leading to the metabolic disorder for calcium absorption and phosphorus metabolism,thus aggravating renal osteodystrophy(P<0.05).
