Comparison of Methods for Generating Thrombotic Models after the Endothelial Injury
10.12259/j.issn.2095-610X.S20240112
- VernacularTitle:内皮损伤后血栓模型制备方法的比较
- Author:
Jingqiu ZHANG
1
;
Yongping LU
;
Linkun TA
;
Yue SUN
;
Qi CHEN
Author Information
1. 云南大学附属医院/云南省第二人民医院超声科,云南 昆明 650021
- Keywords:
Rabbit;
Endothelial injury;
Venous thrombosis;
Real-time shear wave elastography
- From:
Journal of Kunming Medical University
2024;45(1):35-40
- CountryChina
- Language:Chinese
-
Abstract:
Objective Endothelial injury plays a crucial role in forming deep vein thrombosis.This study aims to compare the effectiveness of various methods for creating rabbit femoral vein thrombotic models after the endothelial injuryso as to provide a solid experimental foundation for further research on the endothelial injury and deep vein thrombosis.Methods Forty-five rabbits were randomly divided into three groups(A,B,C),with 15 cases in each group and subjected to the endothelial injury using the methods of simple clamping,combined complete ligation,and combined incomplete ligation,respectively.The intravascular ultrasonic manifestations and local endothelial pathological changes were compared at 1,3,and 7 days after modeling.Results Significant differences in vascular diameter and Young's modulus values were observed after 7 days of modeling(P<0.05).In pairwise comparisons between the groups,the Young's modulus values in group C were significantly higher than those in groups A and B after 7 days of modeling(P<0.05).Pathological examination confirmed the presence of fibr-inoid thrombus in the blood vessels of group C on the seventh day of modeling.Conclusion Combining simple clam-ping and incomplete ligation can produce a relatively stable endothelial injury and thrombus formation.This method provides a robust experimental model for further investigation into deep vein thrombosis after the endothelial injury.
