Role of Mertk-mediated NF-κb Pathway in Inflammatory Response of Schwann Cells
10.12259/j.issn.2095-610X.S20231203
- VernacularTitle:Mertk介导NF-κb通路影响雪旺细胞炎症反应的作用
- Author:
Yidan FU
1
;
Wenting CHEN
;
Xiaoyang SU
;
Yan ZHAO
;
Danfeng LAN
;
Qiuping YANG
Author Information
1. 昆明医科大学第一附属医院干疗科,云南 昆明 650032
- Keywords:
Mertk;
Ikbkb;
Nuclear factor kappa-b;
Schwann cells;
Diabetes peripheral neuropathy
- From:
Journal of Kunming Medical University
2023;44(12):20-24
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the regulatory effect of Mertk expression level on NF-κb pathway in rat Schwann cells and its possible mechanism.Methods Rat Schwann cells were cultured in vitro,and the expression of Mertk in Schwann cells exposed to high glucose was detected by Western blot.Co-immunoprecipitation was used to detect the interaction between endogenous Mertk and Ikbkb.Western blot was used to detect the expression levels of Ikbkb,P65 and tumor necrosis factor-α in Schwann cells after Mertk silencing.The protein expressions of Mertk,Ikbkb and P65 after silencing Mertk were detected by immunofluorescence.Results Mertk was expressed in Schwann cells,and the expression level increased with the increase of glucose concentration.Co-immunoprecipitation assay showed that Mertk interacted with Ikbkb in rat Schwann cells.Compared with the control group,the expression level of Mertk was significantly decreased(P<0.05),while Ikbkb,P65 and TNF-α were significantly increased(P<0.05)after knock down expression of Mertk in Schwann cells.Immunofluorescence experiments showed that the fluorescence of Mertk was decreased,and the fluorescence of Ikbkb and P65 was increased in the silenced Schwann cells.Conclusion After the expression of Mertk is decreased,it can mediate the regulation of NF-κb pathway in Schwann cells through interaction with Ikbkb,and up-regulate the expression of P65 and inflammatory factor TNF-α.
