Effect of Median Nerve Electrical Stimulation on Synaptic Plasticity in Ischemic Stroke Rats
10.12259/j.issn.2095-610X.S20231201
- VernacularTitle:正中神经电刺激对缺血性脑卒中大鼠突触可塑性的影响
- Author:
Chengcai ZHANG
1
;
Rong NING
;
Na CHEN
;
Yichen PENG
;
Li ZHOU
;
Xichen YANG
;
Jingyi LU
;
Pengyue ZHANG
;
Rui LI
Author Information
1. 云南中医药大学第二临床医学院,云南 昆明 650500
- Keywords:
Median nerve electrical stimulation;
Ischemic stroke;
Synaptic plasticity
- From:
Journal of Kunming Medical University
2023;44(12):6-12
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects and mechanisms of median nerve electrical stimulation on synaptic plasticity in ischemic stroke rats.Methods 18 healthy male SD rats were randomly divided into Sham group(n = 6),ischemic stroke group(MCAO group,n = 6)and median nerve electrical stimulation group(MNES group,n = 6).The left middle cerebral artery occlusion model of rats was established by thread plug method.Thread plug was not inserted in sham group.The median nerve electrical stimulation group was given median nerve electrical stimulation intervention on the 3rd day after modeling,and intervention on the next day.After intervention for 7 times,behavioral detection,HE staining was used to detect median nerve injury.Nissl staining was used to detect cerebral infarction volume.Western blot was used for detection of the expression level of proteins related to synaptic plasticity,and electron microscopy was performed.Results HE staining showed that median nerve electrical stimulation did not cause damage to the median nerve in stroke rats,and the median nerve membrane was intact without obvious inflammatory cells.Compared with MCAO group,the neural function,motor function and coordination of the injured forelimb in MNES group were significantly improved(P<0.01).Compared with MCAO group,cerebral infarction volume in MNES group was significantly reduced(P<0.05),the pyknosis of Nissl bodies in ischemic penumbra decreased.Compared with MCAO group,the expression levels of synaptic plastication-related proteins PSD95 and synI in the cortex of MNES group were significantly up-regulated after median nerve electrical stimulation(P<0.05),the number of synapses in the ischemic cortex increased significantly(P<0.01).Conclusion Median nerve electrical stimulation is a safe and effective therapeutic measure to improve nerve function after stroke,and its mechanism is related to promoting synaptic plasticity.
