Development and evaluation of mild TBI rapid diagnostic strips based on GFAP labeled with quantum dots
10.7644/j.issn.1674-9960.2024.02.006
- VernacularTitle:基于量子点标记胶质纤维酸性蛋白的轻度颅脑损伤快速诊断试纸条研制及评价
- Author:
Changle YU
1
;
Wenliang FU
;
Weiwei XING
;
Donggang XU
Author Information
1. 军事科学院军事医学研究院军事认知与脑科学研究所,北京 100850
- Keywords:
mild traumatic brain injury;
quantum dot;
glial fibrillary acid protein;
immunochromatographic test strip;
rapid diagnosis
- From:
Military Medical Sciences
2024;48(2):115-121
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish an auxiliary method for diagnosis of mild traumatic brain injury based on serum GFAP rapid detection test strips using immunochromatographic technology labeled with quantum dot microspheres.Methods The quantum dot microspheres were coupled with GFAP antibodies.The detection conditions were optimized to obtain the fluorescence probe in order to prepare the immunochromatographic test strips.An auxiliary diagnostic method was established after optimization of detection conditions.Finally,the auxiliary diagnostic effect of the test strips was evaluated using clinical samples.Results The serum concentration of GFAP could be detected by the optimized test strips within 13 mins with a detection limit of 0.15 ng/mL,and no more than 70μL of the serum sample was required.In addition,good reproducibility was achieved by different batches of test strips(CV=10.7%).The detection sensitivity and specificity of the strips to mild traumatic brain injury using 51 clinical samples were 95.24%and 96.67%respectively,indicating good effects of detection.Conclusion The developed test strips are user-friendly with reliable results,which can facilitate field rapid diagnosis of mild traumatic brain injury in complicated wartime environments.
