Identification and diagnostic value of characteristic volatile organic compounds in exhaled breath of mice with early stage radiation injury
10.7644/j.issn.1674-9960.2024.02.002
- VernacularTitle:辐射损伤早期小鼠呼出气特征性挥发性有机化合物分析及诊断价值
- Author:
Bingkun XIAO
1
;
Xiaoyao MIAO
;
Zhiheng LI
;
Xiaofei ZHENG
;
Rongqing HUANG
Author Information
1. 军事科学院军事医学研究院辐射医学研究所,北京 100850
- Keywords:
volatile organic compounds;
radiation injury;
exhaled breath;
thermal desorption gas chromatography-mass spectrometry;
diagnostic model
- From:
Military Medical Sciences
2024;48(2):88-94
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the characteristic volatile organic compounds(VOCs)in exhaled breath and their diagnostic value in mice with early stage radiation injury.Methods The thermal desorption gas chromatography-mass spectrometry(TD-GC/MS)technique was used to analyze VOCs in exhaled breath of irradiated mice by 60Coγ-ray with 800 cGy.The characteristic VOCs in the early stage of radiation injury were identified,and a diagnostic model was established.Results The 30-day survival rate of mice was 4.2%.There were significant differences in characteristic VOCs at 7 hours after radiation injury,and thirty characteristic VOCs related to early-stage radiation injury were identified.The diagnostic value of differential metabolites in mice after irradiation was evaluated via the ROC curve,and the area under the ROC curve(AUC)of a single compound exceeded 0.8.The diagnostic model was constructed by screening 9 potential biomarkers of exhalation through Fisher discriminant analysis,and its sensitivity and specificity were close to 100%.Conclusion Analysis of VOCs in exhaled breath is expected to provide a non-invasive diagnostic method for early screening and diagnosis of radiation injury.
