An immunochromatographic method for SARS-CoV-2 based on the assistance of internal reference protein
10.7644/j.issn.1674-9960.2023.12.006
- VernacularTitle:基于内参辅助的新型冠状病毒荧光免疫层析方法
- Author:
Ziyi WANG
1
;
Xiaodan CHENG
;
Xingsheng YANG
;
Zhen RONG
;
Shengqi WANG
Author Information
1. 军事科学院军事医学研究院生物信息中心,北京 100850
- Keywords:
SARS-CoV-2;
internal reference protein;
immunochromatography;
quantum dots;
fluorescence
- From:
Military Medical Sciences
2023;47(12):913-918
- CountryChina
- Language:Chinese
-
Abstract:
Objective To monitor the validity of the sample process flow and to improve the detection accuracy of a SARS-CoV-2 antigen by introducing internal reference into SARS-CoV-2 fluorescent lateral flow assay.Methods Silica-core double-layer quantum dots(SiO2-DQD)microspheres were prepared using a polyethyleneimine(PEI)-mediated self-assembly method before being separately conjugated with an internal reference detection antibody and SARS-CoV-2 antibody.Their capture antibodies were plotted on two test lines of the nitrocellulose membrane.Based on double-antibody sandwich detection principles,an internal reference-assisted fluorescent lateral flow assay for SARS-CoV-2 antigen was established.Results According to the readout fluorescent signals,the detection limit of the method for the SARS-CoV-2 antigen reached 0.01 ng/ml with a validated sample process,suggesting its good specificity and stability.Conclusion In this study,a rapid internal reference-integrated fluorescent lateral flow assay for SARS-CoV-2 has been established,which provides control reference for the analysis workflow so that the false-negative rate of the test results can be reduced.
