Scutellarin inhibitting BV-2 microglia-mediated neuroinflammation via the cyclic GMP-AMP synthase-stimulator of interferon gene pathway
10.16098/j.issn.0529-1356.2024.02.002
- VernacularTitle:灯盏乙素通过环状GMP-AMP合酶-干扰素基因刺激因子通路抑制BV-2小胶质细胞介导的神经炎症
- Author:
Zhao-Da DUAN
1
;
Li YANG
;
Hao-Lun CHEN
;
Teng-Teng LIU
;
Li-Yang ZHENG
;
Dong-Yao XU
;
Chun-Yun WU
Author Information
1. 昆明医科大学基础医学院人体解剖学与组织学胚胎学系,昆明 650500
- Keywords:
Scutellarin;
BV-2 microglia;
Cyclic GMP-AMP synthetase-stimulator of interferon gene pathway;
PYD domains-containing protein 3;
Neuroinflammation;
Immunofluorescence;
Western blotting
- From:
Acta Anatomica Sinica
2024;55(2):133-142
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of scutellarin on lipopolysaccharide(LPS)induced neuroinflammation in BV-2 microglia cells.Methods BV-2 microglia were cultured and randomly divided into 6 groups:control group(Ctrl),cyclic GMP-AMP synthetase(cGAS)inhibitor RU320521 group(RU.521 group),LPS group,LPS+RU.521 group,LPS+scutellarin pretreatment group(LPS+S)and LPS+S+RU.521 group.The expressions of cGAS,stimulator of interferon gene(STING),nuclear factor kappa B(NF-κB),phosphorylated NF-κB(p-NF-κB),neuroinflammatory factors PYD domains-containing protein 3(NLRP3)and tumor necrosis factor α(TNF-α)in BV-2 microglia were detected by Western blotting and immunofluorescent double staining(n= 3).Results Western blotting and immunofluorescent double staining showed that compared with the control group,the expression of cGAS,STING,p-NF-κB,NLRP3 and TNF-α in BV-2 microglia increased significantly after LPS induction(P<0.05),while the expression of cGAS,STING,p-NF-κB,NLRP3 and TNF-α in LPS+S group were significantly lower than those in LPS group(P<0.05).Treatment with cGAS pathway inhibitor RU.521 showed similar effects as the pre-treatment group with scutellarin.In addition,the change of NF-κB in each group was not statistically significant(P>0.05).Conclusion Scutellarin inhibits the neuroinflammation mediated by BV-2 microglia cells,which may be related to cGAS-STING signaling pathway.
