The Effect of Indomethacin on Na-K-ATPase and K-pNPPase Activity of Rat Brain.
- Author:
Ki Uk KIM
1
;
Young Tae KIM
;
Sang Yong KIM
;
Young Woo LEE
Author Information
1. Department of Neurosurgery, Yonsei University, College of Medicine, Seoul, Korea.
- Publication Type:Original Article
- Keywords:
Indomethacin;
Na-K-ATPase;
K-pNPPase;
Lineweaver-Burk plot;
K+- dependent dephosphorylation
- MeSH:
Adenosine Triphosphate;
Animals;
Brain*;
Indomethacin*;
Rats*;
Sodium
- From:Journal of Korean Neurosurgical Society
1986;15(3):511-520
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
The effect of indomethacin on Na-K-ATPase and K-pNPPase activity was studied with rat brain homogenate. The results were as follows : 1) Indomethacin inhibited both Na-K-ATPase and K-pNPPase in a dose-related pattern. 2) Inhibition mode of indomethacin for K+ in both Na-K-ATPase and K-pNPPase was competitive type. 3) Indomethacin showed stimulative effect at lower sodium concentration below 10mM, and showed inhibitory effect at higher sodium concentration on Na-K-ATPase and K-pNPPase activity, and the inhibitory effect was slightly increased with increasing concentration of sodium. 4) The inhibitory effect of indomethacin on Na-K-ATPase activity was increased with increasing ATP concentration, but was not affected by Mg++ concentration. These results indicate that indomethacin inhibits Na-K-ATPase activity by inhibiting K+- dependent dephosphorylation steps.
