AP2M1 inhibits proliferation and invasion of diffuse large B-cell lymphoma cells
10.16352/j.issn.1001-6325.2024.03.0308
- VernacularTitle:AP2M1抑制弥漫性大B细胞淋巴瘤细胞增殖和侵袭
- Author:
Yuanchun LI
1
;
Xueqian YAN
;
Dan FAN
;
Yueru JI
;
Fang XIAO
;
Jing GAO
Author Information
1. 空军军医大学第二附属医院 血液内科,陕西 西安 710001
- Keywords:
diffuse large B-cell lymphoma;
adaptor related protein complex 2 subunit μ1(AP2M1);
epidermal growth factor recep-tor;
phosphatidylinositol 3-kinase;
protein kinase B
- From:
Basic & Clinical Medicine
2024;44(3):308-316
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the regulatory effect of the adaptor related protein complex 2 subunit μ1(AP2M1)on proliferation and invasion of diffuse large B-cell lymphoma(DLBCL).Methods Human diffuse large B-cell lymphoma cell line OCI-LY8 was aliquoted into control group,NC-shRNA group,AP2M1-shRNA group,NC-LV group,and AP2M1-LV group.Lipofectamine 2000 was used for cell transfection.Cell proliferation was detected by tetramethylazolium salt(MTT)method,apoptosis was detected by flow cytometry and cell migration and invasion were detected by Transwell assay.The protein expression of AP2M1,epidermal growth factor receptor(EGFR),p-phosphatidylinositol 3 kinase(PI3K),PI3K,p-protein kinase B(Akt)and AKT was detec-ted by Western blot.Results Compared with control group,the relative expression of AP2M1 mRNA and protein in the AP2M1-shRNA group was decreased(P＜0.05).The relative cell viability was increased(P＜0.05).The cell apoptosis rate was decreased(P＜0.05).The counting number of migrating and invading cells was in-creased(P＜0.05).The relative expression level of EGFR protein and the phosphorylation level of PI3K and AKT were increased(P＜0.05).Compared with Control group,the expression of AP2M1 mRNA and protein relative ex-pression level in AP2M1-LV group was increased(P＜0.05).The relative cell viability was decreased(P＜0.05).The cell apoptosis rate was increased(P＜0.05).The number of migrating and invading cells was decreased(P＜0.05).The relative expression level of EGFR protein and the phosphorylation level of PI3K and AKT were all decreased(P＜0.05).Conclusions The over-expression of AP2M1 partially inhibits the proliferation and invasion of DLBCL cells by inhibiting the EGFR/PI3K/AKT signaling pathway.
