FHL2 regulates THP-1 macrophage foaming through NF-κB signaling pathway
10.16352/j.issn.1001-6325.2024.02.0204
- VernacularTitle:FHL2通过NF-κB信号通路调节THP-1巨噬细胞泡沫化
- Author:
Weiwei CHEN
1
;
Huang LIAO
;
Zhenhong SHI
;
Ying LUO
Author Information
1. 海南医学院第二附属医院 心血管内科,海南 海口 570216
- Keywords:
four-and-a-half LIM-only protein 2(FHL2);
NF-κB signaling pathway;
THP-1;
macrophage foaming
- From:
Basic & Clinical Medicine
2024;44(2):204-209
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine whether four-and-a-half LIM-only protein 2(FHL2)can affect macrophage foa-ming by regulating nuclear factor kappa-B(NF-κB)signaling pathway.Methods FHL2 over-expression plasmids and siRNA of FHL2 were constructed and transfected into human monocyte/macrophages cell line THP-1.Western blot was used to detect the expression of FHL2.The cells were stimulated with oxidized low density lipoprotein(ox-LDL)and the expression of IL-6,IL-1β,TNF-α and other cytokines were detected by ELISA.Oil red O staining was used to detect the degree of cell foaming.The protein expression of NF-κB signaling pathway was detected by Western blot.Results The expression of FHL2 increased after transfected with FHL2 over-expression plasmids while decreased in si-FLH2 transfected cells.FHL2 down-regulated secretion of inflammatory cytokines.Down-regulation of FHL2 allevi-ated THP-1 macrophage foaming.The down-regulation of FHL2 inhibited activation of NF-κB signaling pathway,while the over-expression FHL2 showed an opposite trend.Conclusions FHL2 down-regulation inhibits the activation of NF-κB signaling pathway,reduces the secretion of inflammatory cytokines and alleviates foaming of macrophages.
