Tetrandrine attenuates IL-1β-induced injury of human articular chondrocytes in vitro
10.16352/j.issn.1001-6325.2024.02.0174
- VernacularTitle:粉防己碱减轻IL-1β诱导的体外人关节软骨细胞损伤
- Author:
Yunlang OUYANG
1
;
Ning ZHANG
;
Liping CHEN
;
Bingshen JIA
;
Tuo JIAO
Author Information
1. 海南医学院第一附属医院 关节外科,海南 海口 570000
- Keywords:
tetrandrine;
osteoarthritis;
interleukin-1β;
articular chondrocytes;
damage
- From:
Basic & Clinical Medicine
2024;44(2):174-179
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of tetrandrine(Tet)on injury of primary human articular chondro-cytes induced by interleukin-1β(IL-1β).Methods Human articular chondrocytes were divided into control group,IL-1β group,hypoxia inducible factor(HIF-1α)inhibitor group[2-methoxyestradiol(2-ME2)group],Tet groups containing low,medium and high concentrations.Each group has six replicated samples.MTT assay was used to de-tect the viability of cells;cell apoptosis was detected by flow cytometry;the level of inflammatory related factors like tumor necrosis factor-α(TNF-α),matrix metalloproteinase 3(MMP-3),inducible nitric oxide synthase(iNOS),cyclooxygenase-2(COX-2)and the activity of antioxidant factors like super oxide dismutase(SOD)and glutathione peroxides(GPx)in cells were detected by ELISA;Western blot was used to detect the expression of HIF-1α and VEGF in cells.Results Compared with the control group,the apoptosis rate,level of TNF-α,MMP-3,iNOS,COX-2 and the protein expression of HIF-1α and VEGF in IL-1β group all increased,the cell survival rate and the activity of SOD and GPx significantly decreased(P＜0.05);compared with IL-1β group,the apoptosis rate,the level of TNF-α,MMP-3,iNOS,COX-2 and the protein expression of HIF-1α and VEGF in 2-ME2 group and Tet groups with low,medium,and high concentration significanthy decreased(P＜0.05).The cell survival rate and the activity of SOD and GPx significantly increased(P＜0.05).Conclusions Tetrandrine attenuates IL-1β-in-duced injury of human articular chondrocytes.
